dc.creatorBlomqvist, Karin 
dc.creatorAlbrecht, Letusa 
dc.creatorQuintana, Maria del Pilar 
dc.creatorAngeletti, Davide 
dc.creatorJoannin, Nicolas 
dc.creatorChêne, Arnaud 
dc.creatorMoll, Kirsten 
dc.creatorWahlgren, Mats 
dc.date.accessioned2014-08-11T14:48:06Z
dc.date.available2014-08-11T14:48:06Z
dc.date.created2013-01
dc.identifier.issnISSN:1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/8765
dc.description.abstractImmunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface
dc.format.mediumRecurso electrónico
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofPLOS ONE, ISSN 1932-6203, V. 8 N. 1 Jan, 2013
dc.relation.urihttp://www.ncbi.nlm.nih.gov.ez.urosario.edu.co/pmc/articles/PMC3546040/pdf/pone.0052679.pdf
dc.sourceinstname:Universidad del Rosario
dc.sourcereponame:Repositorio Institucional EdocUR
dc.titleA Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
dc.typearticle
dc.audienceComunidad Rosarista
dc.publisherUniversidad del Rosario
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.subject.decsMalaria
dc.subject.decsInmunología
dc.subject.decsPfEMP1
dc.subject.decsPlasmodium
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto completo)
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.format.tipoDocumento
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.
dc.creator.googleBlomqvist, Karin
dc.creator.googleAlbrecht, Letusa
dc.creator.googleQuintana, Maria del Pilar
dc.creator.googleAngeletti, Davide
dc.creator.googleJoannin, Nicolas
dc.creator.googleChêne, Arnaud
dc.creator.googleMoll, Kirsten
dc.creator.googleWahlgren, Mats


Archivos en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

 

Reconocimientos: