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Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

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dc.creatorRangel, Nelson
dc.creatorVillegas Gálvez, Victoria Eugenia
dc.creatorRondón-Lagos, Milena
dc.creator.googleRangel, Nelsonspa
dc.creator.googleVillegas, Victoria E
dc.creator.googleRondón-Lagos, Milena
dc.date.accessioned2018-11-19T21:31:11Z
dc.date.available2018-11-19T21:31:11Z
dc.date.created2017
dc.date.issued2017
dc.description.abstractOne area of great importance in breast cancer (BC) research is the study of gene expression regulated by both estrogenic and antiestrogenic agents. Although many studies have been performed in this area, most of them have only addressed the effects of 17β-estradiol (E2) and tamoxifen (TAM) on MCF7 cells. This study aimed to determine the effect of low doses of E2 and TAM on the expression levels of 84 key genes, which are commonly involved in breast carcinogenesis, in four BC cell lines differentially expressing estrogen receptor (ER) α and HER2 (MCF7, T47D, BT474, and SKBR3). The results allowed us to determine the expression patterns modulated by E2 and TAM in ERα+ and ERα− cell lines, as well as to identify differences in expression patterns. Although the MCF7 cell line is the most frequently used model to determine gene expression profiles in response to E2 and TAM, the changes in gene expression patterns identified in ERα+ and ERα− cell lines could reflect distinctive properties of these cells. Our results could provide important markers to be validated in BC patient samples, and subsequently used for predicting the outcome in ERα+ and ERα− tumors after TAM or hormonal therapy. Considering that BC is a molecularly heterogeneous disease, it is important to understand how well, and which cell lines, best model that diversity. © 2017 Rangel et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.issnISSN 1179-1314
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18708
dc.language.isoengspa
dc.relation.citationEndPage550
dc.relation.citationStartPage537
dc.relation.citationTitleBreast Cancer: Targets and Therapy
dc.relation.citationVolumeVol. 9
dc.relation.ispartofBreast Cancer: Targets and Therapy, ISSN: 1179-1314, Vol. 9 (2017) pp. 537-550spa
dc.relation.urihttps://www.dovepress.com/getfile.php?fileID=38497spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by-nc/3.0/spa
dc.source.bibliographicCitationFerlay, J., Soerjomataram, I., Ervik, M., (2013) GLOBOCAN 2012 V1.0, Cancer Incidence and Mortality Worldwide, , http://globocan.iarc.fr, IARC cancer base no. 11 [Internet]. Lyon: IARCspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject17Β-Estradiolspa
dc.subjectBreast Cancerspa
dc.subjectCell Linesspa
dc.subjectErα+spa
dc.subjectErα−spa
dc.subjectQpcrspa
dc.subjectTamoxifenspa
dc.subject.decsEstradiolspa
dc.subject.decsMucin 1spa
dc.subject.decsTamoxifenspa
dc.subject.decsApoptosisspa
dc.subject.decsArticlespa
dc.subject.decsBc Cell Linespa
dc.subject.decsBirc5 Genespa
dc.subject.decsCarcinogenesisspa
dc.subject.decsCell Culturespa
dc.subject.decsCell Cycle Progressionspa
dc.subject.decsCell Proliferationspa
dc.subject.decsCell Survivalspa
dc.subject.decsCell Transformationspa
dc.subject.decsControlled Studyspa
dc.subject.decsDna Damagespa
dc.subject.decsDna Fingerprintingspa
dc.subject.decsEstrogen Receptor Negative Breast Cancerspa
dc.subject.decsEstrogen Receptor Positive Breast Cancerspa
dc.subject.decsGenespa
dc.subject.decsGene Expressionspa
dc.subject.decsHumanspa
dc.subject.decsHuman Cellspa
dc.subject.decsMcf-7 Cell Linespa
dc.subject.decsMetastasisspa
dc.subject.decsMucin 1 Genespa
dc.subject.decsReal Time Polymerase Chain Reactionspa
dc.subject.decsReverse Transcription Polymerase Chain Reactionspa
dc.subject.lembNeoplasmasspa
dc.subject.lembCarcinogénesisspa
dc.subject.lembTamoxifenospa
dc.titleProfiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell linesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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