Ítem
Acceso Abierto

In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes

Mostrar el registro sencillo de la publicación
dc.creatorRojas-Caraballo, Josespa
dc.creatorLópez-Abán, Juliospa
dc.creatorPérez del Villa, Luisspa
dc.creatorVizcaíno, Carolinaspa
dc.creatorVicente, Belénspa
dc.creatorFernández-Soto, Pedrospa
dc.creatordel Olmo, Estherspa
dc.creatorAlfonso Patarroyo, Manuelspa
dc.creatorMuro, Antoniospa
dc.creator.googleRojas-Caraballo, Josespa
dc.creator.googleLópez-Abán, Juliospa
dc.creator.googlePérez del Villa, Luisspa
dc.creator.googleVizcaíno, Carolinaspa
dc.creator.googleVicente, Belénspa
dc.creator.googleFernández-Soto, Pedrospa
dc.creator.googleAlfonso Patarroyo, Manuelspa
dc.creator.googleMuro, Antoniospa
dc.date.accessioned2018-11-07T16:00:25Z
dc.date.available2018-11-07T16:00:25Z
dc.date.created2014
dc.date.issued2014
dc.description.abstractFasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p<0.05) and a mixed Th1/Th2/Th17/Treg immune response, according to IFN-γ, IL-4, IL-17 and IL-10 levels, accompanied by increased CD62L+ T-cell populations. A high level of protection was obtained in mice vaccinated with peptides B2, B5, B6 and T15 formulated in the ADAD vaccination system with the AA0029 immunomodulator. The bioinformatics approach used in the present study led to the identification of seven peptides as vaccine candidates against the infection caused by Fasciola hepatica (a liver-fluke trematode). However, vaccine efficacy must be evaluated in other host species, including those having veterinary importance. © 2014 Rojas-Caraballo et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0105323
dc.identifier.issn1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18685
dc.language.isoengspa
dc.relation.citationEndPage19
dc.relation.citationIssueNo. 8
dc.relation.citationStartPage1
dc.relation.citationTitlePloS one
dc.relation.citationVolumeVol. 9
dc.relation.ispartofPloS one, ISSNe 1932-6203, Vol. 9/No. 8 (2014);pp. 1-19spa
dc.relation.urihttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0105323&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitationKaplan, R.M., Fasciola hepatica: A review of the economic impact in cattle and considerations for control (2001) Vet Ther, 2, pp. 40-50spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.decsFasciola hepáticaspa
dc.subject.decsEnzimologíaspa
dc.subject.decsParasitologíaspa
dc.subject.keywordCytokinespa
dc.subject.keywordAnimaleng
dc.subject.keywordT-Lymphocyteeng
dc.subject.keywordB-Lymphocyteeng
dc.subject.keywordEpitopespa
dc.subject.keywordHelminth Antibodyspa
dc.subject.keywordImmunoglobulin Gspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtozoal Vaccinespa
dc.subject.keywordAmino Acid Sequencespa
dc.subject.keywordAnimalspa
dc.subject.keywordBloodspa
dc.subject.keywordChemistryspa
dc.subject.keywordCluster Analysisspa
dc.subject.keywordDisease Modelspa
dc.subject.keywordFasciola Hepaticaspa
dc.subject.keywordFascioliasisspa
dc.subject.keywordFemalespa
dc.subject.keywordGene Expression Profilingspa
dc.subject.keywordGeneticsspa
dc.subject.keywordImmunologyspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMortalityspa
dc.subject.keywordMousespa
dc.subject.keywordParasitologyspa
dc.subject.keywordSynthesisspa
dc.subject.keywordAmino Acid Sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordDisease Modelseng
dc.subject.keywordEpitopeseng
dc.subject.keywordEpitopeseng
dc.subject.keywordFasciola Hepaticaspa
dc.subject.keywordFascioliasisspa
dc.subject.keywordGene Expression Profilingspa
dc.subject.keywordMicespa
dc.subject.keywordPeptidesspa
dc.subject.keywordProtozoan Vaccinesspa
dc.titleIn vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
111.pdf
Tamaño:
2 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos