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A trans-methylation mechanism between the two major H3K9 methyltransferases SETDB1 and SUV39H1 regulates heterochromatin establishment

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dc.contributor.advisorAit-Si-Ali, Slimane
dc.contributor.advisorRamírez Clavijo, Sandra Rocío
dc.creatorCruz Tapias, Paola Andrea
dc.creator.degreeDoctor en Ciencias Biomédicasspa
dc.creator.degreetypeFull timespa
dc.date.accessioned2019-06-04T20:50:36Z
dc.date.available2019-06-04T20:50:36Z
dc.date.created2018-09-21
dc.date.issued2018
dc.descriptionLa tri-metilación de la lisina 9 de la histona 3 (H3K9me3) es una modificación epigenética requerida para la formación y el mantenimiento de la heterocromatina, la estabilidad genómica y el silenciamiento de elementos transposables en células madre embrionarias (CMEs). SETDB1 es una metiltransferasa específica de la lisina 9 de la histona 3 crucial durante el desarrollo de los mamíferos debido a que regula la pluripotencia de las CMEs en el embrión. Los resultados de este trabajo sugieren que SETDB1 lleva a cabo un proceso de auto-metilación que es requerido para el mantenimiento de la pluripotencia, el crecimiento y la viabilidad de las CMEs murinas. Adicionalmente, análisis de transcriptoma completo (RNA-seq) mostraron que la integridad de las dos lisinas auto-metiladas es requerida para el silenciamiento tanto de genes codificantes como de elementos transposables. De hecho, análisis de ChIP-seq revelaron que una deficiencia en la auto-metilación conlleva a una disminución en el establecimiento de H3K9me3 en loci blanco. Nuestros resultados sugieren que la auto-metilación de SETDB1 es un pre-requisito para la trans-metilación por SUV39H1. Este mecanismo podría regular no solamente la interacción física entre SETDB1 y SUV39H1 (vía el cromodominio de SUV39H1), sino también la cooperación para el establecimiento y el mantenimiento de la heterocromatina y el silenciamiento de los elementos transposables. Por todo lo anterior, los resultados de este trabajo revelan un nuevo mecanismo que regula las funciones de SETDB1, el cual es crucial para la identidad y el mantenimiento de las CMEs.spa
dc.description.abstractHistone H3 lysine 9 trimethylation (H3K9me3) is a key epigenetic modification required for heterochromatin formation and maintenance, genome stability and silencing of transposable elements in embryonic stems cells (ESCs). The H3K9-specific methyltransferase (KMT) SETDB1 is vital for mammalian development as it regulates ESCs pluripotency in the early embryo. Here we unravel that SETDB1 undergoes automethylation on two lysines, embedded within its catalytic domain, both in vitro and in cells. Importantly, SETDB1 automethylation is required for mouse ESCs stemness, growth and viability. Hence, transcriptome-wide analyses (RNA-seq) show that the integrity of the two SETDB1 automethylated lysines is required for both coding genes and transposable elements silencing in mESCs. Indeed, our analyses of ChIP-seq show that automethylation-deficient SETDB1 expression leads to a lack of H3K9me3 establishment at target loci. Interestingly, our results point to a model in which SETDB1 auto-methylation paves the path to a subsequent trans-methylation by SUV39H1. This mechanism could regulate not only the SETDB1/SUV39H1 physical interaction (via the SUV39H1 chromodomain), but also cooperation in the establishment and maintenance of both heterochromatin blocks (large domains) and transposable elements silencing. Taken together, my findings uncover a novel mechanism regulating SETDB1 KMT key functions that are key in embryonic stems cells identity maintenance.spa
dc.description.embargo2021-06-05 01:01:01: Script de automatizacion de embargos. info:eu-repo/date/embargoEnd/2021-06-04
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_19828
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19828
dc.language.isoeng
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentFacultad de Ciencias Naturales y Matemáticasspa
dc.publisher.programDoctorado en Ciencias Biomédicasspa
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombiaspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
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dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectEpigénesisspa
dc.subjectGenéticaspa
dc.subject.ddcEvolución & genéticaspa
dc.subject.keywordSETDB1spa
dc.subject.keywordSUV39H1spa
dc.subject.keywordLysine methylationspa
dc.subject.keywordPost-translational modificationspa
dc.subject.keywordEmbryonic stems cellsspa
dc.subject.lembSETDB1spa
dc.subject.lembSUV39H1spa
dc.subject.lembMetilación de lisinasspa
dc.subject.lembModificaciones post-traduccionalesspa
dc.subject.lembCélulas madre embrionariasspa
dc.titleA trans-methylation mechanism between the two major H3K9 methyltransferases SETDB1 and SUV39H1 regulates heterochromatin establishmenteng
dc.title.TranslatedTitleUn mecanismo de transmetilación entre las dos principales metiltransferasas H3K9 SETDB1 y SUV39H1 regula el establecimiento de heterocromatinaspa
dc.title.TranslatedTitleUn mécanisme de trans-méthylation entre les deux principales méthyltransférases de H3K9 SETDB1 et SUV39H1, régule l'établissement de l'hétérochromatinefre
dc.typedoctoralThesiseng
dc.type.documentTrabajo de gradospa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTesis de doctoradospa
local.department.reportEscuela de Medicina y Ciencias de la Saludspa
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Doctorado en Ciencias Biomédicas