Ítem
Acceso Abierto

On the evolution and function of Plasmodium vivax reticulocyte binding surface antigen (pvrbsa)

Mostrar el registro sencillo de la publicación
dc.creatorCamargo-Ayala, Paola Andrea
dc.creatorGarzón-Ospina, Diego
dc.creatorMoreno Pérez, Darwin Andrés
dc.creatorRicaurte-Contreras, Laura Alejandra
dc.creatorNoya, Oscar
dc.creatorPatarroyo, Manuel A.
dc.creator.googleCamargo-Ayala, Paola Andreaspa
dc.creator.googleGarzón-Ospina, Diegospa
dc.creator.googleMoreno Pérez, Darwin Andrésspa
dc.creator.googleRicaurte-Contreras, Laura Alejandraspa
dc.creator.googleNoya, Oscarspa
dc.creator.googlePatarroyo, Manuel A.spa
dc.date.accessioned2019-02-12T19:57:27Z
dc.date.available2019-02-12T19:57:27Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractThe RBSA protein is encoded by a gene described in Plasmodium species having tropism for reticulocytes. Since this protein is antigenic in natural infections and can bind to target cells, it has been proposed as a potential candidate for an anti-Plasmodium vivax vaccine. However, genetic diversity (a challenge which must be overcome for ensuring fully effective vaccine design) has not been described at this locus. Likewise, the minimum regions mediating specific parasite-host interaction have not been determined. This is why the rbsa gene's evolutionary history is being here described, as well as the P. vivax rbsa (pvrbsa) genetic diversity and the specific regions mediating parasite adhesion to reticulocytes. Unlike what has previously been reported, rbsa was also present in several parasite species belonging to the monkey-malaria clade; paralogs were also found in Plasmodium parasites invading reticulocytes. The pvrbsa locus had less diversity than other merozoite surface proteins where natural selection and recombination were the main evolutionary forces involved in causing the observed polymorphism. The N-terminal end (PvRBSA-A) was conserved and under functional constraint; consequently, it was expressed as recombinant protein for binding assays. This protein fragment bound to reticulocytes whilst the C-terminus, included in recombinant PvRBSA-B (which was not under functional constraint), did not. Interestingly, two PvRBSA-A-derived peptides were able to inhibit protein binding to reticulocytes. Specific conserved and functionally important peptides within PvRBSA-A could thus be considered when designing a fully-effective vaccine against P. vivax. © 2018 Camargo-Ayala, Garzón-Ospina, Moreno-Pérez, Ricaurte-Contreras, Noya and Patarroyo.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.3389/fgene.2018.00372
dc.identifier.issn1664-8021
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19050
dc.language.isoengspa
dc.relation.citationTitleFrontiers in Genetics
dc.relation.citationVolumeVol. 9
dc.relation.ispartofFrontiers in Genetics, ISSN:1664-8021, Vol. 9 (2018)spa
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fgene.2018.00372/fullspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationAbascal, F., Zardoya, R., Telford, M.J., TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations (2010) Nucleic Acids Res, 38 (WEB SERVER ISSUE), pp. W7-W13spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectBlood Analysisspa
dc.subjectChromatographyspa
dc.subjectDna Extractionspa
dc.subjectEscherichia Colispa
dc.subjectEvolutionspa
dc.subjectGene Mutationspa
dc.subjectGenetic Polymorphismspa
dc.subjectGenetic Transformationspa
dc.subjectGenetic Variabilityspa
dc.subjectHumanspa
dc.subjectMajor Clinical Studyspa
dc.subjectMicroscopyspa
dc.subjectPlasmidspa
dc.subjectPlasmodium Vivaxspa
dc.subjectPolymerase Chain Reactionspa
dc.subjectReticulocytespa
dc.subjectSequence Analysisspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordArticlespa
dc.subject.lembAnálisis de sangrespa
dc.subject.lembCromatografíaspa
dc.subject.lembADNspa
dc.titleOn the evolution and function of Plasmodium vivax reticulocyte binding surface antigen (pvrbsa)spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
14.pdf
Tamaño:
1.55 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos