@mastersthesis{10336/20148,
author = {Cubides Amézquita, Jenner Rodrigo},
year = {2019},
url = {http://repository.urosario.edu.co/handle/10336/20148},
abstract = {Even though several malaria vaccine candidates are currently in clinical phase 2, and 3 trials, their mechanisms of immune protection are not fully understood, and durations of vaccine protection are largely unknown. The Aotus monkey model has been recommended by the World Health Organization for the study of malaria vaccine candidates because of strong similarities to pathology and genetic background observed in humans.  However, the lack of antibodies specific for molecular surface markers of immune cells in Aotus have delayed advances in malaria research.  Among commercial monoclonal antibodies (mAbs) for human CD19+, CD27+ B cell and CD4+, CD45RO+ memory T cell molecular markers, only those of the clone SK3 recognized Aotus CD4 T cells. Here, bioinformatics approaches were used to design antigenic peptides that correspond to the extracellular regions of the membrane proteins CD19, CD27, CD4, and CD45RO, to produce mAbs. 1746 resulting hybridoma clones recognized molecular surface markers of immune cells by flow cytometry and 30% of them bond to the synthetic peptide by ELISA. The mAbs, CD194G12A3G3, CD275F11C11, CD45H3D10, and CD45RO3A8G1 bound to 17.7%, 40.1%, 27.4 and 51% of the Aotus’ PBMCs with high affinity (100 ng/106 cells) but displayed only medium affinity to human cells (300 ng/106 cells) in FACS analyses. Double staining of B and T cells showed that the mAbs CD194G12A3G3 and CD275F11C11 recognized 15.9%, and CD45H3D10 and CD45RO3A8G1 bound to 20.6% of Aotus’ PBMCs, suggesting that the mAbs recognized membrane protein markers of memory B and T cells. These mAbs are useful for the identification and tracking of memory cells in the Aotus model to elucidate which human immune cells may mediate protection against malaria},
keywords = {Inmunología},
keywords = {Anticuerpo Monoclonal},
keywords = {Citometría de flujo},
title = {Producción de anticuerpos monoclonales que reconozcan proteínas de membrana en células de memoria en aotus spp},
publisher = {Universidad del Rosario},
}