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- ÍtemAcceso Abierto16/6-idiotype expressing antibodies induce brain inflammation and cognitive impairment in mice: the mosaic of central nervous system involvement in lupus(2013-04-04) Shaye, Kivity; Katzav, Aviva; Arango, María-Teresa; Landau-Rabi, Moran; Zafrir, Yaron; Agmon-Levin, Nancy; Blank, Miri; Anaya, Juan-Manuel; Mozes, Edna; Chapman, Joab; Shoenfeld, YehudaBackground: The 16/6-idiotype (16/6-Id) of the human anti-DNA antibody was found to induce experimental lupus in naive mice, manifested by production of autoantibodies, leukopenia and elevated inflammatory markers, as well as kidney and brain involvement. We assessed behavior and brain pathology of naive mice injected intracerebra-ventricularly (ICV) with the 16/6-Id antibody. Methods: C3H female mice were injected ICV to the right hemisphere with the human 16/6-Id antibody or commercial human IgG antibodies (control). The mice were tested for depression by the forced swimming test (FST), locomotor and explorative activity by the staircase test, and cognitive functions were examined by the novel object recognition and Y-maze tests. Brain slices were stained for inflammatory processes. Results: 16/6-Id injected mice were cognitively impaired as shown by significant differences in the preference for a new object in the novel object recognition test compared to controls (P = 0.012). Similarly, the preference for spatial novelty in the Y-maze test was significantly higher in the control group compared to the 16/6-Id-injected mice (42% vs. 9%, respectively, P = 0.065). Depression-like behavior and locomotor activity were not significantly different between the16/6-Id-injected and the control mice. Immunohistochemistry analysis revealed an increase in astrocytes and microglial activation in the hippocampus and amygdala, in the 16/6-Id injected group compared to the control. Conclusions: Passive transfer of 16/6-Id antibodies directly into mice brain resulted in cognitive impairments and histological evidence for brain inflammation. These findings shed additional light on the diverse mosaic pathophysiology of neuropsychiatric lupus.
- ÍtemAcceso Abierto1607. Una hipótesis sobre la formación de Estado en los Andes Colombianos(2006-10) Chaparro Amaya, AdolfoActualmente es un lugar común de la filosofía política reconocer una violencia anterior, un exceso de fuerza que legitima y antecede toda fundación de Estado. En el caso colombiano, no está claro cómo y por qué el acontecimiento mismo de la fundación borra las huellas de esa violencia para hacer visible sólo el Estado-institución (Colonia), o para magnificar los ritos y los emblemas aglutinadores del Estado-nación (República). Nuestra pretensión es abordar el problema desde la investigación de la génesis del Estado en la Nueva Granada, con la intuición de que el diagrama básico de configuración estatal surgido en el siglo XVI es pertinente en un análisis actual del Estado-nación en Colombia.
- ÍtemAcceso Abierto20 años de la caída del Muro de Berlín(2010-01-01) Leiteritz, Ralf JuanHace veinte años era un adolescente en último año de bachillerato en Leipzig, la segunda ciudad más grande de Alemania Oriental, a unos 150 kilómetros al sur de Berlín. Pasé mi niñez y cursé mi educación preuniversitaria en la República Democrática Alemana; en otras palabras, detrás de la llamada “Cortina de Hierro”. Mi educación estuvo dominada por la ideología socialista y fuertemente controlada por el Partido Comunista. El currículum presentaba una interpretación restringida de la historia a través del lente teórico del marxismo-leninismo. Materias como “Staatsbürgerkunde”, literalmente “currículum ciudadano”, tenían como fin implantar el dogma comunista en los cerebros de los estudiantes. Viéndolo desde el exterior, Alemania Oriental debió de haberse visto como la realización de la visión de George Orwell en 1984.
- ÍtemSolo Metadatos20 años de la caída del Muro de Berlín(2010-01-01) Leiteritz, Ralf JuanHace veinte años era un adolescente en último año de bachillerato en Leipzig, la segunda ciudad más grande de Alemania Oriental, a unos 150 kilómetros al sur de Berlín. Pasé mi niñez y cursé mi educación preuniversitaria en la República Democrática Alemana; en otras palabras, detrás de la llamada “Cortina de Hierro”. Mi educación estuvo dominada por la ideología socialista y fuertemente controlada por el Partido Comunista. El currículum presentaba una interpretación restringida de la historia a través del lente teórico del marxismo-leninismo. Materias como “Staatsbürgerkunde”, literalmente “currículum ciudadano”, tenían como fin implantar el dogma comunista en los cerebros de los estudiantes. Viéndolo desde el exterior, Alemania Oriental debió de haberse visto como la realización de la visión de George Orwell en 1984.
- ÍtemAcceso Abierto20. Opinión pública y proceso de paz en Colombia en 2014(2016) Charry Joya, Carlos AndrésEste artículo presenta los principales eventos sobre el proceso de paz entre el gobierno colombiano y la guerrilla de las FARC durante 2014. Esta presentación se hizo dando una especial atención de las diferentes formas de difusión e interpretación del proceso expuesto por los medios de comunicación. En este trabajo se analizan los medios de comunicación como un actor esencial del proceso de construcción de la opinión pública; por esa razón, su punto de vista es una clave para entender los diálogos de paz, así como el desarrollo del conflicto armado en Colombia.
- ÍtemSolo Metadatos22q11.2 DS syndrome as a genetic subtype of schizophrenia(2015) Huertas-Rodríguez, Cindy Katherin; Payan-Gomez, Cesar; Forero-Castro, Ruth MaribelIntroduction The 22q11.2 deletion syndrome (22q11.2 DS) is associated with the microdeletion of this chromosomal region, and represents the second most common genetic syndrome after Down's syndrome. In patients with schizophrenia, 22q11.2 DS has a prevalence of 2%, and in selected groups can be increased to between 32-53%. Objective To describe the generalities of 22q11.2 DS syndrome as a genetic subtype of schizophrenia, its clinical characteristics, molecular genetic aspects, and frequency in different populations. Methods A review was performed from 1967 to 2013 in scientific databases, compiling articles about 22q11.2 DS syndrome and its association with schizophrenia. Results The 22q11.2 DS syndrome has a variable phenotype associated with other genetic syndromes, birth defects in many tissues and organs, and a high rate of psychiatric disorders, particularly schizophrenia. Likewise, it has been identified in clinical populations with schizophrenia selected by the presence of common syndromic characteristics. FISH, qPCR and MLPA techniques, and recently, aCGH and NGS technologies, are being used to diagnose this microdeletion. Conclusions It is important in clinical practice to remember that people suffering the 22q11.2 DS have a high genetic risk for developing schizophrenia, and it is considered that the simultaneous presence of this disease and 22q11.2 DS represents a genetic subtype of schizophrenia. There are clear phenotypic criteria, molecular and cytogenetic methods to diagnose this group of patients, and to optimize a multidisciplinary approach in their monitoring. © 2014 Asociación Colombiana de Psiquiatría.
- ÍtemSolo Metadatos273 ANTIEPILEPTIC DRUGS IN PAIN CAUSED BY DIABETIC NEUROPATHY(2007-06) Gutierrez?Alvarez, A.M.; Beltran?Rodriguez, J.A.; Moreno, C.B.Pain is frequent in diabetic neuropathy and is hard tomanage. Antiepileptic drugs have been used in treatingpain for several decades now. Nevertheless, their e?ec-tiveness when used as analgesics in painful diabetic neu-ropathy still remains controversial. We conducted ameta-analysis for determining which antiepileptic drughad the best analgesic potential for managing pain inpatients su?ering from painful diabetic neuropathy. Thesearch covered the Cochrane, MEDLINE, EMBASEand LILAC S databases, between January 1966 and Sep-tember 2005.
- ÍtemAcceso Abierto3D Analysis of the TCR/pMHCII Complex Formation in Monkeys Vaccinated with the First Peptide Inducing Sterilizing Immunity against Human Malaria(2010) Patarroyo, Manuel A.; Bermudez, Adriana; López, Carolina; Yepes, Gloria; Patarroyo, Manuel E.T-cell receptor gene rearrangements were studied in Aotus monkeys developing high antibody titers and sterilizing immunity against the Plasmodium falciparum malaria parasite upon vaccination with the modified synthetic peptide 24112, which was identified in the Merozoite Surface Protein 2 (MSP-2) and is known to bind to HLA-DR beta 1*0403 molecules with high capacity. Spectratyping analysis showed a preferential usage of V beta 12 and V beta 6 TCR gene families in 67% of HLA-DR beta 1*0403-like genotyped monkeys. Docking of peptide 24112 into the HLA-DR beta 1*0401-HA peptide-HA1.7TCR complex containing the VDJ rearrangements identified in fully protected monkeys showed a different structural signature compared to nonprotected monkeys. These striking results show the exquisite specificity of the TCR/pMHCII complex formation needed for inducing sterilizing immunity and provide important hints for a logical and rational methodology to develop multiepitopic, minimal subunit-based synthetic vaccines against infectious diseases, among them malaria.
- ÍtemSolo Metadatos3D structure and immunogenicity of Plasmodium falciparum sporozoite induced associated protein peptides as components of fully-protective anti-malarial vaccine(2011) Alba M.P.; Almonacid H.; Calderón D.; Chacón E.A.; Poloche L.A.; Patarroyo M.A.; Patarroyo M.E.SIAP-1 and SIAP-2 are proteins which are implicated in early events involving Plasmodium falciparum infection of the Anopheles mosquito vector and the human host. High affinity HeLa and HepG2 cell binding conserved peptides have been previously identified in these proteins, i.e. SIAP-1 34893 ( 421KVQGLSYLLRRKNGTKHPVY 440) and SIAP-1 34899 ( 541YVLNSKLLNSRSFDKFKWIQ 560) and SIAP-2 36879 ( 181LLLYSTNSEDNLDISFGELQ 200). When amino acid sequences have been properly modified (replacements shown in bold) they have induced high antibody titres against sporozoites in Aotus monkeys (assessed by IFA) and in the corresponding recombinant proteins (determined by ELISA and Western blot). 1H NMR studies of these conserved native and modified high activity binding peptides (HABPs) revealed that all had ?-helical structures in different locations and lengths. Conserved and corresponding modified HABPs displayed different lengths between the residues fitting into MHCII molecule pockets 1-9 and different amino acid orientation based on their different HLA-DR?1 * binding motifs and binding registers, suggesting that such modifications were associated with making them immunogenic. The results suggested that these modified HAPBs could be potential targets for inclusion as components of a fully-effective, minimal sub-unit based, multi-epitope, and multistage anti-malarial vaccine. © 2011 Elsevier Inc..
- ÍtemAcceso Abierto3D structure determination of STARP peptides implicated in P. falciparum invasion of hepatic cells(2010) Bermudez, Adriana; Alba, Martha Patricia; Vanegas, Magnolia; Patarroyo, Manuel ElkinTo block the different stages of Plasmodium falciparum invasion into human hepatocytes and red blood cells, we have focused on those proteins belonging to the pre-erythrocytic stage. One of these proteins is Sporozoite Threonine and Asparagine Rich Protein (STARP), which is a ligand used by P. falciparum parasites to bind Hepatic cells (HepG2). Previous studies on this protein identified two conserved peptides binding with high activity to HepG2 cells (namely 20546 and 20570) with corresponding critical hepatic-cell binding residues and determined an important role for these two peptides in the invasion process. This study shows the results of immunization trials in Aotus monkeys with native STARP peptides and analogues modified in critical hepatic-cell binding residues. The results show that native peptides are not immunogenic but can induce high-antibody titers when their critical residues are replaced by other with similar volume and mass but different polarity. Nuclear Magnetic Resonance (1H NMR) studies revealed that native peptides (non-immunogenic) displayed shorter ?-helical regions compared to their highly immunogenic modified analogues. Binding assays with HLA-DR?1* molecules showed that 20546 modified peptides inducing high-antibody titers (24972, 24320 and 24486) bound to HLA-DR?1*0301 molecules, while the 20570 modified analogue (24322) bound to HLA-DR?1*0101. The results support including these high-immunogenic STARP-derived modified peptides as pre-erythrocytic candidates to be included in the design of a synthetic antimalarial vaccine. © 2010 Elsevier Ltd.
- ÍtemSolo Metadatos433 Palynostratigraphy of the eocene esmeraldas formation, middle Magdalena Valley Basin, Colombia(2012) Rodriguez, Guillermo; Oboh-Ikuenobe, Francisca; Jaramillo, Carlos; Rueda, Milton; Cadena, Edwin AlbertoThe Eocene EsmeraldasFormation isa very importantoil reservoir intheMiddle Magdalena ValleyBasin, Colombia, which is a preduct of the fragmentatienof a Cenozoic forelandbasinby the uplift of the Northem Andes. Ihe lateralcentinuity of the formation,as well as its correlations with lithostratigraphic units inadjacent basins isnot clearly understood. The Los CorrosFossilHorizon,a molluscan horizonin the upper partof the Esmeraldas Formation,has been used to tracethe top of the formation, Thishorizonisnot laterallycontinuous over thebasinand its age is still debatable. Some of the key palynostratigraphic events inNorthernSouth America are net present inthepalynological record of the Esmeraldas, Palynologicaldata from 82 samples from an eutcrop section intheNuevo Mundo Synclinearea and from seven previouslystudied wells have been integratedwith a palynologicalzonation of nonhem South America inorder to datctheEsrneraldasForrnation.The age ranges from the lateEarly Eocene to the Late Eocene. The Esmeraldas Forrnationiscorrelative withthe upper Picacho ForTnation and the lowerpartof the ConcentracionForrnationin the EasternCoTdillera, and theupper Mirador Forrnationand thebaseof theCarboneraFormationintheLlanosFeothills. [[heLos Corros FossilHorizon is Late Eocene and is time-correlative with a marine transgression inthe central Llanos Foothills,A non-menic multidimensional scaling analysis suggests that florasfrom the Middle Magdalena Valleywere differentfromthe Llanos Foothillsarea during the Middle to Late Eocene. This is apparently due to taphonomic effects. The results efthis study conuibute to a betterunderstanding of theoverall evolution ef the Middle Magdalena ValleyBasin and the evolution of thenonhem Andes.
- ÍtemSolo Metadatos5-alpha-reductase inhibitors for lower urinary tract symptoms secondary to benign prostatic obstruction(2015) García?Perdomo, Herney A; Lopez, Hugo E; Tacklind, JamesThis is the protocol for a review and there is no abstract. The objectives are as follows: To assess the effects of 5-alpha-reductase inhibitors (5ARIs) for treating lower urinary tract symptoms (LUTS) secondary to benign prostatic obstruction (BPO). © 2015 The Cochrane Collaboration.
- ÍtemAcceso Abierto50 Jahre FARC: Geschichtsbild und Selbstverständnis der ältesten Guerilla Lateinamerikas(2015) Schuster, Sven BenjamínIm Mai 1964 erstürmte die kolumbianische Armee im Rahmen des sogenannten Plan LASO (Latin American Security Operation) die von Kommunisten gegründete Agrarkolonie Marquetalia im Süden Kolumbiens. Die sich über mehrere Monate erstreckende Militäroperation gegen die zwischen den Departements Huila und Tolima gelegene Enklave wurde von den USA logistisch unterstützt, wobei Tausende Soldaten, Hubschrauber sowie mit Brandbomben bestückte Kampfflugzeuge zum Einsatz kamen. Für die US-Regierung unter Präsident Lyndon B. Johnson war es im Kontext der bereits von Kennedy initiierten Alliance for Progress von größter Bedeutung, ein „zweites Kuba“ in Südamerika zu verhindern. Neben materiellen Hilfsleistungen und Kooperationen in den Bereichen der medizinischen Versorgung, dem Wohnungsbau und dem Bildungswesen war der Aspekt der counterinsurgency ein Kernstück des dezidiert antikommunistischen Programms.1 Trotz des massiven Aufgebots war der Militärschlag jedoch ein Misserfolg. So gelang den in Marquetalia ansässigen 48 kommunistischen Guerilleros sowie den in ihrem Einflussbereich lebenden Campesinos größtenteils die Flucht. Am Ende hatten die Guerilleros lediglich drei Tote zu beklagen, während sich die Zahl gefallener Soldaten auf über 300 belief.
- ÍtemAcceso Abierto52 Genetic Loci Influencing Myocardial Mass(2016) van der Harst, Pim; van Setten, Jessica; Verweij, Niek; Vogler, Georg; Franke, Lude; Maurano, Matthew T.; Wang, Xinchen; Leach, Irene Mateo; Eijgelsheim, Mark; Sotoodehnia, Nona; Hayward, Caroline; Sorice, Rossella; Meirelles, Osorio; Lyytikäinen, Leo-Pekka; Polašek, Ozren; Tanaka, Toshiko; Arking, Dan E.; Ulivi, Sheila; Trompet, Stella; Müller-Nurasyid, Martina; Smith, Albert V.; Dörr, Marcus; Kerr, Kathleen F.; Magnani, Jared W.; Del Greco M., Fabiola; Zhang, Weihua; Nolte, Ilja M.; Silva, Claudia T.; Padmanabhan, Sandosh; Tragante, Vinicius; Esko, Tõnu; Abecasis, Gonçalo R.; Adriaens, Michiel E.; Andersen, Karl; Barnett, Phil; Bis, Joshua C.; Bodmer, Rolf; Buckley, Brendan M.; Campbell, Harry; Cannon, Megan V.; Chakravarti, Aravinda; Chen, Lin Y.; Delitala, Alessandro; Devereux, Richard B.; Doevendans, Pieter A.; Dominiczak, Anna F.; Ferrucci, Luigi; Ford, Ian; Gieger, Christian; Harris, Tamara B.; Haugen, Eric; Heinig, Matthias; Hernandez, Dena G.; Hillege, Hans L.; Hirschhorn, Joel N.; Hofman, Albert; Hubner, Norbert; Hwang, Shih-Jen; Iorio, Annamaria; Kähönen, Mika; Kellis, Manolis; Kolcic, Ivana; Kooner, Ishminder K.; Kooner, Jaspal S.; Kors, Jan A.; Lakatta, Edward G.; Lage, Kasper; Launer, Lenore J.; Levy, Daniel; Lundby, Alicia; Macfarlane, Peter W.; May, Dalit; Meitinger, Thomas; Metspalu, Andres; Nappo, Stefania; Naitza, Silvia; Neph, Shane; Nord, Alex S.; Nutile, Teresa; Okin, Peter M.; Olsen, Jesper V.; Oostra, Ben A.; Penninger, Josef M.; Pennacchio, Len A.; Pers, Tune H.; Perz, Siegfried; Peters, Annette; Pinto, Yigal M.; Pfeufer, Arne; Pilia, Maria Grazia; Pramstaller, Peter P.; Prins, Bram P.; Raitakari, Olli T.; Raychaudhuri, Soumya; Rice, Ken M.; Rossin, Elizabeth J.; Rotter, Jerome I.; Schafer, Sebastian; Schlessinger, David; Schmidt, Carsten O.; Sehmi, Jobanpreet; Silljé, Herman H.W.; Sinagra, Gianfranco; Sinner, Moritz F.; Slowikowski, Kamil; Soliman, Elsayed Z.; Spector, Timothy D.; Spiering, Wilko; Stamatoyannopoulos, John A.; Stolk, Ronald P.; Strauch, Konstantin; Tan, Sian-Tsung; Tarasov, Kirill V.; Trinh, Bosco; Uitterlinden, Andre G.; van den Boogaard, Malou; van Duijn, Cornelia M.; van Gilst, Wiek H.; Viikari, Jorma S.; Visscher, Peter M.; Vitart, Veronique; Völker, Uwe; Waldenberger, Melanie; Weichenberger, Christian X.; Westra, Harm-Jan; Wijmenga, Cisca; Wolffenbuttel, Bruce H.; Yang, Jian; Bezzina, Connie R.; Munroe, Patricia B.; Snieder, Harold; Wright, Alan F.; Rudan, Igor; Boyer, Laurie A.; Asselbergs, Folkert W.; van Veldhuisen, Dirk J.; Stricker, Bruno H.; Psaty, Bruce M.; Ciullo, Marina; Sanna, Serena; Lehtimäki, Terho; Wilson, James F.; Bandinelli, Stefania; Alonso, Alvaro; Gasparini, Paolo; Jukema, J. Wouter; Kääb, Stefan; Gudnason, Vilmundur; Felix, Stephan B.; Heckbert, Susan R.; de Boer, Rudolf A.; Newton-Cheh, Christopher; Hicks, Andrew A.; Chambers, John C.; Jamshidi, Yalda; Visel, Axel; Christoffels, Vincent M.; Isaacs, Aaron; Samani, Nilesh J.; de Bakker, Paul I.W.Background Myocardial mass is a key determinant of cardiac muscle function and hypertrophy. Myocardial depolarization leading to cardiac muscle contraction is reflected by the amplitude and duration of the QRS complex on the electrocardiogram (ECG). Abnormal QRS amplitude or duration reflect changes in myocardial mass and conduction, and are associated with increased risk of heart failure and death. Objectives This meta-analysis sought to gain insights into the genetic determinants of myocardial mass. Methods We carried out a genome-wide association meta-analysis of 4 QRS traits in up to 73,518 individuals of European ancestry, followed by extensive biological and functional assessment. Results We identified 52 genomic loci, of which 32 are novel, that are reliably associated with 1 or more QRS phenotypes at p and lt; 1 × 10?8. These loci are enriched in regions of open chromatin, histone modifications, and transcription factor binding, suggesting that they represent regions of the genome that are actively transcribed in the human heart. Pathway analyses provided evidence that these loci play a role in cardiac hypertrophy. We further highlighted 67 candidate genes at the identified loci that are preferentially expressed in cardiac tissue and associated with cardiac abnormalities in Drosophila melanogaster and Mus musculus. We validated the regulatory function of a novel variant in the SCN5A/SCN10A locus in vitro and in vivo. Conclusions Taken together, our findings provide new insights into genes and biological pathways controlling myocardial mass and may help identify novel therapeutic targets. © 2016 American College of Cardiology Foundation
- ÍtemAcceso Abierto5azadC treatment upregulates miR-375 level and represses HPV16 E6 expression(2017-05-02) Morel, Adrien; Baguet, Aurélie; Perrard, Jérôme; Demeret, Caroline; Jacquin, Elise; Guenat, David; Mougin, Christiane; Prétet, Jean-LucHigh-risk human papillomaviruses are the etiological agents of cervical cancer and HPV16 is the most oncogenic genotype. Immortalization and transformation of infected cells requires the overexpression of the two viral oncoproteins E6 and E7 following HPV DNA integration into the host cell genome. Integration often leads to the loss of the E2 open reading frame and the corresponding protein can no longer act as a transcriptional repressor on p97 promoter. Recently, it has been proposed that long control region methylation also contributes to the regulation of E6/E7 expression. To determine which epigenetic mechanism is involved in HPV16 early gene regulation, 5-aza-2?-deoxycytidine was used to demethylate Ca Ski and SiHa cell DNA. Decreased expression of E6 mRNA and protein levels was observed in both cell lines in an E2-independent manner. E6 repression was accompanied by neither a modification of the main cellular transcription factor expression involved in long control region regulation, nor by a modification of the E6 mRNA splicing pattern. In contrast, a pronounced upregulation of miR-375, known to destabilize HPV16 early viral mRNA, was observed. Finally, the use of miR-375 inhibitor definitively proved the involvement of miR-375 in E6 repression. These results highlight that cellular DNA methylation modulates HPV16 early gene expression and support a role for epigenetic events in high-risk HPV associated-carcinogenesis.
- ÍtemAcceso AbiertoEl 9 de abril en Cali: cambio social, poder y liminalidad en el Valle del Cauca(2006) Charry Joya, Carlos AndrésA partir de los principios de la sociología procesual de Norbert Elias sobre las relaciones entre grupos sociales establecidos y marginados, y de los referentes analíticos expuestos por Victor Turner acerca de los estados liminales, se analizan en este artículo las implicaciones de los sucesos del 9 de Abril en Cali y el Valle del Cauca. En este sentido, se busca identificar las formas de configuración social de poder a nivel regional y el impacto que tuvo este acontecimiento histórico en tal proceso, donde se destaca una ampliación y mayor posicionamiento de los grupos sociales marginados en el campo social, a consecuencia de las transformaciones sociopolíticas acaecidas en la década de 1930 y, en especial, en la segunda mitad de la década de 1940, por la incidencia del gaitanismo y la denominada resistencia civil. Con el asesinato de Jorge Eliécer Gaitán se aprecia un cambio considerable en los equilibrios de poder entre sectores sociales, que contraviene la tendencia antes reseñada. Se considera que los elementos de constitución del campo simbólico son indispensables para la comprensión del proceso de cambio, así como de las formas sociales de estructuración del poder.
- ÍtemSolo Metadatos99 mTc-mebrofenin hepatobiliary scintigraphy in a patient with a bilio-pleural-bronchial fistula(2017) Arevalo-Leal, S.This is a 37 year old patient with a history of cryptogenic cirrhosis on liver transplant waiting list
- ÍtemAcceso AbiertoA administração pública nas sociedades da informação e do conhecimento(2013-01) Valencia Tello, Diana Carolina; Diniz Lima, Edilson VitorelliNa atualidade, a internet e as redes sociais estão mudando radicalmente as formas de interação entre os indivíduos nas sociedades da informação e do conhecimento, razão pela qual os estados e a administração pública em todos os níveis devem se preparar para enfrentar essa nova realidade, procurando responder às demandas da cidadania, que busca maior participação, transparência e efetividade do Estado. Nesse contexto, um dos elementos mais importantes para viabilizar a participação do cidadão nas decisões públicas que afetam a comunidade é o acesso à informação pública. Mas em burocracias de tipo weberiano, dominadas por interesses políticos e econômicos, a informação é protegida, entre outras razões, para evitar a crítica da sociedade. Para desenvolver melhor esse problema, analisaremos inicialmente a administração pública nas sociedades da informação e do conhecimento; no segundo item apresentaremos a burocracia em Weber e no terceiro item estudaremos as características da burocracia na América Latina. No quarto momento será tratado especificamente o caso brasileiro, com ênfase para o problema atual da divulgação dos gastos da Copa do Mundo de 2014. Finalmente, no quinto segmento se exporá o programa de governo eletrônico na Colômbia.
- ÍtemSolo MetadatosA B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes(2005-11) López, Ramsés; Urquiza, Mauricio; Patino, Helena; Suárez, Jorge; Reyes, Claudia; Patarroyo, Manuel A.; Patarroyo, Manuel E.Epstein–Barr virus (EBV) infects human target cells mainly through gp350/220-CD21 and gp42-MHCII interactions; however, it has been shown that these interactions are dispensable for EBV-invasion of susceptible cells, suggesting that other viral proteins are involved in this process. It is probable that tegument BNRF1/p140 protein is involved in EBV-invasion of target cells, since anti-p140 antibodies inhibit EBV-infection of B-lymphocytes and there is evidence that part of the protein is located on virus surface. Sixty-six peptides, covering the entire BNRF1/p140 sequence, were synthesised and tested in lymphoblastoid cell line binding assays. Peptides 11465 and 11521 bound with high affinity to Raji, Ramos and P3HR-1 cells but not to erythrocytes, showing cell-binding behaviour similar to EBV. These two peptides induced antibodies recognising live EBV-infected cells. Interestingly, peptide-11521 (YVLQNAHQIACHFHSNGTDA) or antibodies induced by this peptide inhibited EBV-binding to B-lymphocytes, suggesting that this p140-region could be involved in EBV and B-lymphocyte interaction.
- ÍtemSolo MetadatosA Bayesian examination of the relationship of internal and external touchpoints in the customer experience process across various service environments(2020) Siqueira, Jose Ribamar; Horst, Enrique ter; Molina, German; Losada, Mauricio; Mateus, Marelby AmadoCustomer experience (CX) is an aggregate of consumer touch points that can be brand-owned (completely controlled by the firm), partner-owned (collectively controlled by the firm and one or more of its partners), customer-owned (where the firm or its partner cannot exert any control or influence) or represented by social/external touch points that account for the importance of the roles of others in the customer experience process. Although the concept of CX is widely used, not much has been examined about how external touch points impact customer perception of experience. Therefore, the main purpose of this study is to examine the role and impact of internal and external touchpoints as dimensions of customer experience and how CX can impact consumer word-of-mouth intention. Service outcome and peace of mind were used to represent internal experience touchpoints and peer to peer quality (PTP) was used to represent an external experience touchpoint. A total of 293 usable surveys were used for analysis using a Bayesian model developed for this study instead of the more traditional SEM approaches. Results showed that an external experience touchpoint has a similar impact to those of internal experience touchpoints in the experience process. CX was also found to have a strong effect on customer word of mouth intention. These findings make various contributions to the literature on CX, especially to research differentiating internal from external experience touchpoints. On the practical side, this study provides recommendations to retailers about how to manage an external touchpoint. © 2019 Elsevier Ltd