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dc.creatorVillegas, Victoria E. 
dc.creatorRahman, Mohammed Ferdous-Ur 
dc.creatorFernandez-Barrena, Maite G. 
dc.creatorDiao, Yumei 
dc.creatorLiapi, Eleni 
dc.creatorSonkoly, Eniko 
dc.creatorStahle, Mona 
dc.creatorPivarcsi, Andor 
dc.creatorAnnaratone, Laura 
dc.creatorSapino, Anna 
dc.creatorRamírez-Clavijo, Sandra 
dc.creatorBurglin, Thomas R. 
dc.creatorShimokawa, Takashi 
dc.creatorRamachandran, Saraswathi 
dc.creatorKapranov, Philipp 
dc.creatorFernandez-Zapico, Martin E. 
dc.creatorZaphiropoulos, Peter G. 
dc.date.accessioned2018-11-21T16:32:41Z
dc.date.available2018-11-21T16:32:41Z
dc.date.created2014
dc.date.issued2014 
dc.identifier.issnISSN 1574-7891
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18719
dc.descriptionNon-coding RNAs are a complex class of nucleic acids, with growing evidence supporting regulatory roles in gene expression. Here we identify a non-coding RNA located head-to-head with the gene encoding the Glioma-associated oncogene 1 (GLI1), a transcriptional effector of multiple cancer-associated signaling pathways. The expression of this three-exon GLI1 antisense (GLI1AS) RNA in cancer cells was concordant with GLI1 levels. siRNAs knockdown of GLI1AS up-regulated GLI1 and increased cellular proliferation and tumor growth in a xenograft model system. Conversely, GLI1AS overexpression decreased the levels of GLI1, its target genes PTCH1 and PTCH2, and cellular proliferation. Additionally, we demonstrate that GLI1 knockdown reduced GLI1AS, while GLI1 overexpression increased GLI1AS, supporting the role of GLI1AS as a target gene of the GLI1 transcription factor. Activation of TGFβ and Hedgehog signaling, two known regulators of GLI1 expression, conferred a concordant up-regulation of GLI1 and GLI1AS in cancer cells. Finally, analysis of the mechanism underlying the interplay between GLI1 and GLI1AS indicates that the non-coding RNA elicits a local alteration of chromatin structure by increasing the silencing mark H3K27me3 and decreasing the recruitment of RNA polymerase II to this locus. Taken together, the data demonstrate the existence of a novel non-coding RNA-based negative feedback loop controlling GLI1 levels, thus expanding the repertoire of mechanisms regulating the expression of this oncogenic transcription factor © 2014 Federation of European Biochemical Societies.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofMolecular Oncology, ISSN: 1574-7891, Vol. 8/No. 5 (2014) pp. 912-926
dc.relation.urihttps://febs.onlinelibrary.wiley.com/doi/epdf/10.1016/j.molonc.2014.03.009
dc.rights.uri
dc.subjectCancer
dc.subjectChromatin
dc.subjectGene Expression
dc.subjectGli1
dc.subjectNon-Coding Rna
dc.subjectTranscription
dc.subject.lembCromatina
dc.subject.lembExpresión genética
dc.subject.lembCáncer
dc.titleIdentification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1
dc.typearticle
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.subject.decsComplementary Rna
dc.subject.decsProtein Patched 1
dc.subject.decsProtein Patched 2
dc.subject.decsRna Polymerase Ii
dc.subject.decsSmall Interfering Rna
dc.subject.decsSonic Hedgehog Protein
dc.subject.decsTranscription Factor Gli1
dc.subject.decsUntranslated Rna
dc.subject.decsChromatin
dc.subject.decsGli Protein
dc.subject.decsOncoprotein
dc.subject.decsRna Polymerase Ii
dc.subject.decsSonic Hedgehog Protein
dc.subject.decsTransactivator Protein
dc.subject.decsTransforming Growth Factor Beta
dc.subject.decsUntranslated Rna
dc.subject.decsTransforming Growth Factor Beta
dc.subject.decsArticle
dc.subject.decsBreast Cancer
dc.subject.decsBreast Fibroadenoma
dc.subject.decsCancer Cell
dc.subject.decsCell Proliferation
dc.subject.decsChromatin Assembly And Disassembly
dc.subject.decsChromatin Immunoprecipitation
dc.subject.decsChromatin Structure
dc.subject.decsChromosome 21Q
dc.subject.decsComparative Study
dc.subject.decsControlled Study
dc.subject.decsDown Regulation
dc.subject.decsExon
dc.subject.decsGene Expression
dc.subject.decsGene Silencing
dc.subject.decsGenetic Transfection
dc.subject.decsHuman
dc.subject.decsHuman Tissue
dc.subject.decsNegative Feedback
dc.subject.decsNucleotide Sequence
dc.subject.decsOncogene
dc.subject.decsPolyadenylation
dc.subject.decsPriority Journal
dc.subject.decsPromoter Region
dc.subject.decsRegulatory Mechanism
dc.subject.decsRna Sequence
dc.subject.decsSignal Transduction
dc.subject.decsTranscription Initiation Site
dc.subject.decsTumor Growth
dc.subject.decsUpregulation
dc.subject.decsChromatin
dc.subject.decsGene Expression Regulation
dc.subject.decsGenetics
dc.subject.decsMetabolism
dc.subject.decsNeoplasm
dc.subject.decsPathology
dc.subject.decsTranscription Initiation
dc.subject.decsTumor Cell Line
dc.subject.decsArticle
dc.subject.decsGenetic Transcription
dc.subject.decsHuman Cell
dc.subject.decsErinaceidae
dc.subject.decsCell Line, Tumor
dc.subject.decsChromatin
dc.subject.decsGene Expression Regulation, Neoplastic
dc.subject.decsHedgehog Proteins
dc.subject.decsHumans
dc.subject.decsNeoplasms
dc.subject.decsOncogene Proteins
dc.subject.decsRna Polymerase Ii
dc.subject.decsRna, Untranslated
dc.subject.decsSignal Transduction
dc.subject.decsTrans-Activators
dc.subject.decsTranscriptional Activation
dc.subject.decsTransforming Growth Factor Beta
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitationBerretta, J., Pinskaya, M., Morillon, A., A cryptic unstable transcript mediates transcriptional trans-silencing of the Ty1 retrotransposon in S. cerevisiae (2008) Genes Dev., 22, pp. 615-626
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.creator.googleVillegas, Victoria E.
dc.creator.googleRahman, Mohammed Ferdous-Ur
dc.creator.googleFernandez-Barrena, Maite G.
dc.creator.googleDiao, Yumei
dc.creator.googleLiapi, Eleni
dc.creator.googleSonkoly, Eniko
dc.creator.googleSonkoly, Eniko
dc.creator.googleRamırez Clavijo, Sandra
dc.creator.googleShimokawa, Takashi
dc.creator.googleRamachandran, Saraswathi
dc.creator.googleKapranov, Philipp
dc.creator.googleFernandez-Zapico, Martin E.
dc.creator.googleZaphiropoulos, Peter G.


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