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dc.creatorBhattacharyya, Tapan 
dc.creatorFalconar, Andrew K. 
dc.creatorLuquetti, Alejandro O. 
dc.creatorCostales, Jaime A. 
dc.creatorGrijalva, Mario J. 
dc.creatorLewis, Michael D. 
dc.creatorMessenger, Louisa A. 
dc.creatorTran, Trang T. 
dc.creatorRamirez, Juan-David 
dc.creatorGuhl, Felipe 
dc.creatorCarrasco, Hernan J. 
dc.creatorDiosque, Patricio 
dc.creatorGarcia, Lineth 
dc.creatorLitvinov, Sergey V. 
dc.creatorMiles, Michael A. 
dc.date.accessioned2018-11-21T19:15:25Z
dc.date.available2018-11-21T19:15:25Z
dc.date.created2014
dc.date.issued2014 
dc.identifier.issnISSN 1935-2727
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18724
dc.descriptionBackground:Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual's history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues.Methodology/Principal Findings:We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology.Conclusions/Significance:These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages. © 2014 Bhattacharyya et al.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 8/No. 5 (2014)
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0002892&type=printable
dc.rights.uri
dc.subjectAntibody
dc.subjectAvidin
dc.subjectBiotin
dc.subjectEpitope
dc.subjectGlycine
dc.subjectMacrogol
dc.subjectParasite Antigen
dc.subjectSynthetic Peptide
dc.subjectEpitope
dc.subjectParasite Antigen
dc.subjectPeptide
dc.subjectProtozoon Antibody
dc.subjectTrypomastigote Small Surface Antigen, Trypanosoma Cruzi
dc.subjectVariant Surface Glycoprotein
dc.subjectAlgorithm
dc.subjectAnimal Experiment
dc.subjectAntibody Response
dc.subjectAntibody Specificity
dc.subjectAntigen Recognition
dc.subjectArticle
dc.subjectBrazil
dc.subjectCell Lineage
dc.subjectCell Lysate
dc.subjectChagas Disease
dc.subjectChronic Disease
dc.subjectComparative Study
dc.subjectControlled Study
dc.subjectEcg Abnormality
dc.subjectEnzyme Linked Immunosorbent Assay
dc.subjectGenotyping Technique
dc.subjectGeographic Distribution
dc.subjectHuman
dc.subjectImmunogenicity
dc.subjectMajor Clinical Study
dc.subjectMouse
dc.subjectNonhuman
dc.subjectNucleotide Sequence
dc.subjectParasite Identification
dc.subjectPeptide Synthesis
dc.subjectPolymerase Chain Reaction
dc.subjectSerology
dc.subjectSouth America
dc.subjectTrypanosoma Cruzi
dc.subjectAmino Acid Sequence
dc.subjectAnimal
dc.subjectBiology
dc.subjectBlood
dc.subjectChagas Disease
dc.subjectChemistry
dc.subjectClassification
dc.subjectImmunology
dc.subjectMolecular Genetics
dc.subjectParasitology
dc.subjectProcedures
dc.subjectSerotyping
dc.subjectTriatoma
dc.subjectAlgorithms
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectAntibodies, Protozoan
dc.subjectAntigens, Protozoan
dc.subjectChagas Disease
dc.subjectComputational Biology
dc.subjectEpitopes
dc.subjectHumans
dc.subjectMice
dc.subjectMolecular Sequence Data
dc.subjectPeptides
dc.subjectSerotyping
dc.subjectSouth America
dc.subjectTriatoma
dc.subjectTrypanosoma Cruzi
dc.subjectVariant Surface Glycoproteins, Trypanosoma
dc.subject.lembEnfermedad de Chagas
dc.subject.lembTrypanosomiasis
dc.subject.lembCardiomiopatía Chagásica
dc.titleDevelopment of peptide-based lineage-specific serology for chronic Chagas disease : Geographical and clinical distribution of epitope recognition
dc.typearticle
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitation(2013) Chagas disease (American trypanosomiasis), , World Health Organization. Fact sheet N°340. Geneva: World Health Organisation
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/
dc.creator.googleBhattacharyya, Tapan
dc.creator.googleFalconar, Andrew K.
dc.creator.googleLuquetti, Alejandro O.
dc.creator.googleCostales, Jaime A.
dc.creator.googleGrijalva, Mario J.
dc.creator.googleLewis, Michael D.
dc.creator.googleTran, Trang T.
dc.creator.googleGuhl, Felipe
dc.creator.googleDiosque, Patricio
dc.creator.googleGarcia, Lineth
dc.creator.googleLitvinov, Sergey V.
dc.creator.googleMiles, Michael A.


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