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dc.creatorde-la-Torre, Alejandra 
dc.creatorSauer, Arnaud 
dc.creatorPfaff, Alexander W. 
dc.creatorBourcier, Tristan 
dc.creatorBrunet, Julie 
dc.creatorSpeeg-Schatz, Claude 
dc.creatorBallonzoli, Laurent 
dc.creatorVillard, Odile 
dc.creatorAjzenberg, Daniel 
dc.creatorSundar, Natarajan 
dc.creatorGrigg, Michael E. 
dc.creatorGomez-Marin, Jorge E. 
dc.creatorCandolfi, Ermanno 
dc.date.accessioned2018-11-22T19:33:16Z
dc.date.available2018-11-22T19:33:16Z
dc.date.created2013
dc.date.issued2013 
dc.identifier.issnISSN 1935-2727
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18733
dc.descriptionIn a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 7/No. 11 (2013)
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0002541&type=printable
dc.rights.uri
dc.subjectGamma Interferon
dc.subjectGranulocyte Colony Stimulating Factor
dc.subjectGranulocyte Macrophage Colony Stimulating Factor
dc.subjectInterleukin 1 Receptor Blocking Agent
dc.subjectInterleukin 1
dc.subjectInterleukin 13
dc.subjectInterleukin 15
dc.subjectInterleukin 17
dc.subjectInterleukin 1Beta
dc.subjectInterleukin 2
dc.subjectInterleukin 4
dc.subjectInterleukin 6
dc.subjectInterleukin 8
dc.subjectMacrophage Inflammatory Protein 1Beta
dc.subjectMonocyte Chemotactic Protein 1
dc.subjectParasite Antibody
dc.subjectTumor Necrosis Factor Alpha
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectAqueous Humor
dc.subjectArticle
dc.subjectClinical Article
dc.subjectControlled Study
dc.subjectCross-Sectional Study
dc.subjectEnzyme Linked Immunosorbent Assay
dc.subjectEye Examination
dc.subjectGenotyping Technique
dc.subjectHuman
dc.subjectImmunoblotting
dc.subjectMultiplex Polymerase Chain Reaction
dc.subjectParasite Load
dc.subjectPattern Recognition
dc.subjectProtein Expression
dc.subjectReal Time Polymerase Chain Reaction
dc.subjectSerotyping
dc.subjectToxoplasma
dc.subjectToxoplasmosis
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectAged, 8 And Over
dc.subjectCross-Sectional Studies
dc.subjectDna, Protozoan
dc.subjectEye
dc.subjectFemale
dc.subjectGenotype
dc.subjectHumans
dc.subjectInterferon-Gamma
dc.subjectInterleukin-13
dc.subjectInterleukin-17
dc.subjectInterleukin-6
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMultilocus Sequence Typing
dc.subjectPolymerase Chain Reaction
dc.subjectToxoplasma
dc.subjectToxoplasmosis, Ocular
dc.subjectYoung Adult
dc.subject.lembToxoplasmosis
dc.subject.lembCitocinas
dc.subject.lembQuimiocinas
dc.titleSevere South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels
dc.typearticle
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitationArevalo, J.F., Belfort Jr., R., Muccioli, C., Espinoza, J.V., Ocular toxoplasmosis in the developing world (2010) Int Ophthalmol Clin, 50, pp. 57-69
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/
dc.creator.googlede-la-Torre, Alejandra
dc.creator.googleSauer, Arnaud
dc.creator.googlePfaff, Alexander W.
dc.creator.googleBourcier, Tristan
dc.creator.googleBrunet, Julie
dc.creator.googleBallonzoli, Laurent
dc.creator.googleVillard, Odile
dc.creator.googleAjzenberg, Daniel
dc.creator.googleSundar, Natarajan
dc.creator.googleGrigg, Michael E.
dc.creator.googleGomez-Marin, Jorge E.
dc.creator.googleCandolfi, Ermanno


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