Spatial distribution, Leishmania species and clinical traits of Cutaneous Leishmaniasis cases in the Colombian army
AuthorPatino, Luz H.
Duque, Maria Clara
In Colombia, the cutaneous leishmaniasis (CL) is the most common manifestation across the army personnel. Hence, it is mandatory to determine the species associated with the disease as well as the association with the clinical traits. A total of 273 samples of male patients with CL were included in the study and clinical data of the patients was studied. PCR and sequencing analyses (Cytb and HSP70 genes) was performed to identify the species and the intra-specific genetic variability. A georeferenced database was constructed to identify the spatial distribution of Leishmania species isolated. The identification of five species of Leishmania that circulate in the areas where army personnel are deployed is described. Predominant infecting Leishmania species corresponds to L. braziliensis (61.1%), followed by Leishmania panamensis (33.5%), with a high distribution of both species at geographical and municipal level. The species L. guyanensis, L. mexicana and L. lainsoni were also detected at lower frequency. We also showed the identification of different genotypes within L. braziliensis and L. panamensis. In conclusion, we identified the Leishmania species circulating in the areas where Colombian army personnel are deployed, as well as the high intra-specific genetic variability of L. braziliensis and L. panamensis and how these genotypes are distributed at the geographic level. © 2017 Patino et al.
Protozoan ; Cutaneous ; Dna Extraction ; Genetic Variability ; Genotype ; Haplotype ; Human ; Insect Bite ; Leishmania Braziliensis ; Leishmania Guyanensis ; Leishmania Panamensis ; Major Clinical Study ; Male ; Patient Safety ; Phylogenetic Tree ; Phylogeny ; Polymerase Chain Reaction ; Sequence Analysis ; Skin Biopsy ; Skin Leishmaniasis ; Chemistry ; Classification ; Colombia ; Dna Sequence ; Genetics ; Isolation And Purification ; Leishmania ; Leishmaniasis ; Microbiology ; Phylogeography ; Soldier ; Topography ; Young Adult ; Protozoal Dna ; Colombia ; Dna ; Genotype ; Leishmania ; Male ; Military Personnel ; Phylogeography ; Polymerase Chain Reaction ; Young Adult ;
- Artículos 
Showing items related by title, author, creator and subject.
Análisis de la susceptibilidad de una línea celular de Aedes aegypti (Diptera: Culicidae) a la infección con Leishmania (L) chagasi y Leishmania (V) braziliensis Bello, Felio J.; Muñoz-Camargo, Carolina; Barreto, AlfonsoSe evaluó la susceptibilidad de los cultivos celulares derivados de tejidos embrionarios de Aedes aegypti a la infección con Leishmania (L) chagasi y Leishmania (V) braziliensis, agentes etiológicos de leishmaniasis visceral ...Artículo. 2010
"Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis" Patino, Luz H.; Muskus, Carlos; Muñoz, Marina; Ramírez, Juan David"Leishmania amazonensis is one of the causative agents of the different forms of cutaneous leishmaniasis present in Latin America. This species has been isolated from humans and animals (canine/feline) in some endemic ...Artículo. 2020
"Major changes in chromosomal somy, gene expression and gene dosage driven by SbIII in Leishmania braziliensis and Leishmania panamensis" Patino, Luz H.; Imamura, Hideo; Cruz-Saavedra, Lissa; Pavia, Paula; Muskus, Carlos; Méndez-Sarmiento, Claudia ; Dujardin, Jean Claude; Ramírez, Juan David"Leishmania braziliensis and Leishmania panamensis are two species clinically and epidemiologically important, among others because of their relative resistance to first-line drugs (antimonials). The precise mechanism ...Artículo. 2019