Show simple item record

dc.creatorPett, Robert D. 
dc.creatorMcCarthy, Neil E. 
dc.creatorLe Dieu, Rifca 
dc.creatorKerr, Jonathan R. 
dc.date.accessioned2019-01-28T19:21:48Z
dc.date.available2019-01-28T19:21:48Z
dc.date.created2016
dc.date.issued2016 
dc.identifier.issnISSN 1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18945
dc.descriptionBackground: Chronic Fatigue Syndrome (CFS/ME) is a complex multisystem disease of unknown aetiology which causes debilitating symptoms in up to 1% of the global population. Although a large cohort of genes have been shown to exhibit altered expression in CFS/ME patients, it is currently unknown whether microRNA (miRNA) molecules which regulate gene translation contribute to disease pathogenesis. We hypothesized that changes in microRNA expression in patient leukocytes contribute to CFS/ME pathology, and may therefore represent useful diagnostic biomarkers that can be detected in the peripheral blood of CFS/ME patients. Methods: miRNA expression in peripheral blood mononuclear cells (PBMC) from CFS/ME patients and healthy controls was analysed using the Ambion Bioarray V1. miRNA demonstrating differential expression were validated by qRT-PCR and then replicated in fractionated blood leukocyte subsets from an independent patient cohort. The CFS/ME associated miRNA identified by these experiments were then transfected into primary NK cells and gene expression analyses conducted to identify their gene targets. Results: Microarray analysis identified differential expression of 34 miRNA, all of which were up-regulated. Four of the 34 miRNA had confirmed expression changes by qRT-PCR. Fractionating PBMC samples by cell type from an independent patient cohort identified changes in miRNA expression in NK-cells, B-cells and monocytes with the most significant abnormalities occurring in NK cells. Transfecting primary NK cells with hsa-miR-99b or hsamiR-330-3p, resulted in gene expression changes consistent with NK cell activation but diminished cytotoxicity, suggesting that defective NK cell function contributes to CFS/ME pathology. Conclusion: This study demonstrates altered microRNA expression in the peripheral blood mononuclear cells of CFS/ME patients, which are potential diagnostic biomarkers. The greatest degree of miRNA deregulation was identified in NK cells with targets consistent with cellular activation and altered effector function. © 2016 Petty et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofPLoS ONE, ISSN: 1932-6203, Vol. 11/No. 3 (2016); pp.1-19
dc.relation.urihttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150904&type=printable
dc.rights.uri
dc.subjectHsa Microrna 126
dc.subjectHsa Microrna 3C
dc.subjectHsa Microrna 33
dc.subjectHsa Microrna 99B
dc.subjectMicrorna
dc.subjectUnclassified Drug
dc.subjectGenetic Marker
dc.subjectMicrorna
dc.subjectMirn126 Microrna, Human
dc.subjectMirn3 Microrna, Human
dc.subjectMirn33 Microrna, Human
dc.subjectMirn99 Microrna, Human
dc.subjectAdult
dc.subjectArticle
dc.subjectCell Fractionation
dc.subjectCells By Body Anatomy
dc.subjectChronic Fatigue Syndrome
dc.subjectClinical Article
dc.subjectCohort Analysis
dc.subjectControlled Study
dc.subjectCytotoxicity
dc.subjectDiagnostic Test Accuracy Study
dc.subjectGene Expression Regulation
dc.subjectGene Targeting
dc.subjectGenetic Transfection
dc.subjectHuman
dc.subjectHuman Cell
dc.subjectLeukocyte
dc.subjectLymphocyte Activation
dc.subjectLymphocyte Function
dc.subjectMicroarray Analysis
dc.subjectMonocyte
dc.subjectNatural Killer Cell
dc.subjectPeripheral Blood Mononuclear Cell
dc.subjectReceiver Operating Characteristic
dc.subjectReference Value
dc.subjectRna Analysis
dc.subjectUpregulation
dc.subjectDna Microarray
dc.subjectFatigue Syndrome, Chronic
dc.subjectGene Expression Profiling
dc.subjectGenetic Marker
dc.subjectGenetics
dc.subjectImmunology
dc.subjectMetabolism
dc.subjectNatural Killer Cell
dc.subjectPathology
dc.subjectFatigue Syndrome, Chronic
dc.subjectGene Expression Profiling
dc.subjectGenetic Markers
dc.subjectHumans
dc.subjectKiller Cells, Natural
dc.subjectLymphocyte Activation
dc.subjectMicrornas
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectUp-Regulation
dc.subject.ddcEnfermedades 
dc.subject.lembSíndrome de fatiga crónico
dc.subject.lembMarcadores bioquímicos
dc.titleMicroRNAs hsa-miR-99b, hsa-miR-330, hsa-miR-126 and hsa-miR-30c : Potential diagnostic biomarkers in natural killer (NK) cells of patients with Chronic Fatigue Syndrome (CFS)/Myalgic Encephalomyelitis (ME)
dc.typearticle
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitation(2002) A Report of the CFS/ME Working Group.
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/
dc.creator.googlePett, Robert D.
dc.creator.googleMcCarthy, Neil E.
dc.creator.googleLe Dieu, Rifca
dc.creator.googleKerr, Jonathan R.


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

 

Reconocimientos: