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dc.creatorNair, Gayatri 
dc.creatorRebolledo, Mauricio 
dc.creatorClinton White, A. 
dc.creatorCrannell, Zachary 
dc.creatorRichards-Kortum, R. Rebecca 
dc.creatorPinilla, A. Elizabeth 
dc.creatorRamírez, Juan David 
dc.creatorLópez, M. Consuelo 
dc.creatorCastellanos-Gonzalez, Alejandro 
dc.date.accessioned2019-02-15T17:19:14Z
dc.date.available2019-02-15T17:19:14Z
dc.date.created2015
dc.date.issued2015 
dc.identifier.issn0002-9637
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19086
dc.descriptionAmebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofAmerican Journal of Tropical Medicine and Hygiene, ISSN: 0002-9637 Vol. 93, No. 3 (2015) pp. 591-595
dc.relation.urihttp://www.ajtmh.org/docserver/fulltext/14761645/93/3/591.pdf?expires=1544545793&id=id&accname=guest&checksum=FFD734F21CCF67D5AEC1F47F5ADC65C6
dc.subjectRecombinase
dc.subjectProtozoal DNA
dc.subjectRecombinase
dc.subjectAmebiasis
dc.subjectBiochemical equipment
dc.subjectControlled study
dc.subjectDiagnostic test
dc.subjectDiagnostic test accuracy study
dc.subjectEntamoeba
dc.subjectEntamoeba dispar
dc.subject.ddcEnfermedades 
dc.subject.lembDisentería amebiana
dc.subject.lembEntamoeba
dc.subject.lembEntamoeba histolytica
dc.titleDetection of Entamoeba histolytica by recombinase polymerase amplification
dc.typearticle
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.creator.googleNair, Gayatri
dc.creator.googleRebolledo, Mauricio
dc.creator.googleClinton White, A.
dc.creator.googleCrannell, Zachary
dc.creator.googleRichards-Kortum, R. Rebecca
dc.creator.googlePinilla, A. Elizabeth
dc.creator.googleRamírez, Juan David
dc.creator.googleLópez, M. Consuelo
dc.creator.googleCastellanos-Gonzalez, Alejandro
dc.identifier.doi10.4269/ajtmh.15-0276


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