Plasmodium vivax in vitro continuous culture : The spoke in the wheel
Moreno‑Pérez, Darwin Andrés
Patarroyo, Manuel A.
Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described. © 2018 The Author(s).
Cd71 Antigen ; Cell Maturation ; Continuous Culture ; Controlled Study ; Genetic Strain ; Host Cell ; Nonhuman ; Parasite Development ; Parasite Growth ; Parasite Isolation ; Parasite Phenomena And Functions ; Parasite Replication ; Parasite Strain ; Plasmodium Vivax ; Protein Function ; Reticulocyte ; Review ; Target Cell ; Animal ; Chemistry ; Culture Medium ; Microbiological Examination ; Parasitology ; Plasmodium Vivax ; Procedures ; Animals ; Culture Media ; Microbiological Techniques ; Parasitology ; Plasmodium Vivax ; Reticulocytes ;
- Artículos 
Showing items related by title, author, creator and subject.
Prevalence and risk factors for intestinal parasitic infections in pregnant women residing in three districts of Bogotá, Colombia Espinosa-Aranzeles, Angela-Fernanda; Radon, Katja; Froeschl, Guenter; Pinzon-Rondon, Angela Maria; Delius, MariaBackground: Intestinal parasitic infections (IPI) lead to significant morbidity and mortality in pediatric and adult populations worldwide. Intestinal parasitism during pregnancy is of interest as it may affect the health ...Artículo. 2018
Plasmodium falciparum merozoite surface protein 6 (MSP-6) derived peptides bind erythrocytes and partially inhibit parasite invasion López, Ramsés; Valbuena, John; Rodríguez, Luis E.; Ocampo, Marisol; Vera, Ricardo; Curtidor, Hernando; Puentes, Alvaro; García, Javier; Ramirez, Luis E.; Patarroyo, Manuel E.This work shows that Plasmodium falciparum merozoite surface protein-6 (MSP-6) peptides specifically bind to membrane surface receptor on human erythrocytes. Three high activity binding peptides (HABPs) were found: peptides ...Artículo. 2006
Prevalence of intestinal parasitic infections in preschool-children from vulnerable neighborhoods in Bogotá Bouwmans, Marlieke C.H; Gaona-Cifuentes, María Antonia; Chenault, Michelene N; Zuluaga, Catalina; Pinzon-Rondon, Angela MariaIntroducción: Infecciones parasitarias intestinales (IPI) son enfermedades tropicales desatendidas, a pesar de que su prevalencia es alta en muchos países en desarrollo. El impacto en la salud pública de los IPI es importante, ...Artículo. 2016