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dc.creatorBermúdez, Maritza 
dc.creatorMoreno‑Pérez, Darwin Andrés 
dc.creatorArévalo-Pinzón, Gabriela 
dc.creatorCurtidor, Hernando 
dc.creatorPatarroyo, Manuel A. 
dc.date.accessioned2019-03-06T15:02:11Z
dc.date.available2019-03-06T15:02:11Z
dc.date.created2018
dc.date.issued2018
dc.identifier.issn1475-2875
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19199
dc.description.abstractUnderstanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described. © 2018 The Author(s).
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofMalaria Journal, ISSN:1475-2875, Vol. 17 (2018)
dc.relation.urihttps://malariajournal.biomedcentral.com/track/pdf/10.1186/s12936-018-2456-5
dc.subjectCd71 Antigen
dc.subjectCell Maturation
dc.subjectContinuous Culture
dc.subjectControlled Study
dc.subjectGenetic Strain
dc.subjectHost Cell
dc.subjectNonhuman
dc.subjectParasite Development
dc.subjectParasite Growth
dc.subjectParasite Isolation
dc.subjectParasite Phenomena And Functions
dc.subjectParasite Replication
dc.subjectParasite Strain
dc.subjectPlasmodium Vivax
dc.subjectProtein Function
dc.subjectReticulocyte
dc.subjectReview
dc.subjectTarget Cell
dc.subjectAnimal
dc.subjectChemistry
dc.subjectCulture Medium
dc.subjectMicrobiological Examination
dc.subjectParasitology
dc.subjectPlasmodium Vivax
dc.subjectProcedures
dc.subjectAnimals
dc.subjectCulture Media
dc.subjectMicrobiological Techniques
dc.subjectParasitology
dc.subjectPlasmodium Vivax
dc.subjectReticulocytes
dc.subject.ddcEnfermedades 
dc.subject.lembMalaria
dc.subject.lembPlasmodium vivax
dc.titlePlasmodium vivax in vitro continuous culture : The spoke in the wheel
dc.typearticle
dc.subject.keywordDevelopment And Aging
dc.subject.keywordGrowth
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitationBrackett, R.G., Cole, G.C., Green, T.J., Jacobs, R.L., In vitro propagation of Plasmodium falciparum for merozoite antigens (1979) Bull World Health Organ, 57, pp. 33-36. , 397007 2395713
dc.creator.googleBermúdez, Maritza
dc.creator.googleMoreno‑Pérez, Darwin Andrés
dc.creator.googleArévalo-Pinzón, Gabriela
dc.creator.googleCurtidor, Hernando
dc.creator.googlePatarroyo, Manuel Alfonso
dc.identifier.doi10.1186/s12936-018-2456-5
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 17


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