Show simple item record

dc.creatorRodriguez, Deisy Carolina 
dc.creatorOcampo, Marisol 
dc.creatorSalazar, Luz Mary 
dc.creatorPatarroyo, Manuel A. 
dc.date.accessioned2019-10-01T13:08:24Z
dc.date.available2019-10-01T13:08:24Z
dc.date.created2018
dc.date.issued2018
dc.identifier.issn2045-8827
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20370
dc.descriptionMany studies about intracellular microorganisms which are important regarding diseases affecting public health have been focused on the recognition of host–pathogen interactions, thereby ascertaining the mechanisms by which the pathogen invades a cell and makes it become its host. Such knowledge enables understanding the immunological response triggered by these interactions for obtaining useful information for developing vaccines and drugs. Quantitative cell infection assay protocols are indispensable regarding studies involving Mycobacterium tuberculosis, which takes the lives of more than 2 million people worldwide every year; however, sometimes these are limited by the pathogen's slow growth. Concerning such limitation, a detailed review is presented here regarding the different methods for quantifying and differentiating an intracellular pathogen, the importance of mycobacteria aggregate dissociation and multiplicity of infection (MOI) in infection assays. The methods’ differences, advantages, and disadvantages are discussed regarding intra and extracellular bacteria (on cell surface) differentiation, current problems are outlined, as are the solutions provided using fluorophores and projections made concerning quantitative infection assays. © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofMicrobiologyOpen, ISSN:2045-8827, Vol. 7 (2018)
dc.relation.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.588
dc.subjectAntigen Presenting Cell
dc.subjectAssay
dc.subjectBacterial Virulence
dc.subjectCell Differentiation
dc.subjectCell Infection Assay
dc.subjectCell Viability
dc.subjectClinical Protocol
dc.subjectColony Forming Unit
dc.subjectCytokine Production
dc.subjectFluorometry
dc.subjectImmune Response
dc.subjectMicroenvironment
dc.subjectMycobacterium Tuberculosis
dc.subjectNonhuman
dc.subjectOpsonization
dc.subjectPhagocytosis
dc.subjectPhagolysosome
dc.subjectPolymerase Chain Reaction
dc.subjectPriority Journal
dc.subjectPromoter Region
dc.subjectProtein Degradation
dc.subjectQuantitative Analysis
dc.subjectReview
dc.subjectSequence Homology
dc.subjectTuberculosis
dc.subjectCélula presentadora de antígeno
dc.subjectInmunoglobulina M
dc.subjectVirulencia bacteriana
dc.subject.ddcEnfermedades 
dc.subject.lembTuberculosis
dc.subject.lembEnfermedades micobacterianas
dc.titleQuantifying intracellular Mycobacterium tuberculosis : An essential issue for in vitro assays
dc.typereview
dc.subject.keywordBacterial quantification
dc.subject.keywordCytometry
dc.subject.keywordFluorescence
dc.subject.keywordInfection assay
dc.subject.keywordTuberculosis
dc.subject.keywordImmunoglobulin M
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaRevisión
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitationAgerer, F., Waeckerle, S., Hauck, C.R., Microscopic quantification of bacterial invasion by a novel antibody-independent staining method (2004) Journal of Microbiological Methods, 59 (1), pp. 23-32. , https://doi.org/10.1016/j.mimet.2004.05.008, #x0026
dc.creator.googleRodriguez, Deisy Carolina
dc.creator.googleOcampo, Marisol
dc.creator.googleSalazar, Luz Mary
dc.creator.googlePatarroyo, Manuel Alfonso
dc.identifier.doi10.1002/mbo3.588


Files in this item

This item appears in the following Collection(s)

Show simple item record

 

Reconocimientos: