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dc.creatorCubides, Juan Ricardo 
dc.creatorCamargo-Ayala, Paola Andrea 
dc.creatorNiño, Carlos Hernando 
dc.creatorGarzón‑Ospina, Diego 
dc.creatorOrtega‑Ortegón, Anggie 
dc.creatorOspina‑Cantillo, Estefany 
dc.creatorOrduz‑Durán, María Fernanda 
dc.creatorPatarroyo, Manuel Elkin 
dc.creatorPatarroyo, Manuel A. 
dc.date.accessioned2019-10-03T12:35:49Z
dc.date.available2019-10-03T12:35:49Z
dc.date.created2018
dc.date.issued2018
dc.identifier.issn1475-2875
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20382
dc.descriptionBackground: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region. Methods: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained. Conclusion: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage. © 2018 The Author(s).
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofMalaria Journal, ISSN:1475-2875, Vol. 17 (2018)
dc.relation.urihttps://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2286-5
dc.subjectMalaria Vaccine
dc.subjectArticle
dc.subjectColombia
dc.subjectCrt O Gene
dc.subjectDhfr Gene
dc.subjectDhps Gene
dc.subjectDisease Surveillance
dc.subjectElectrophoresis
dc.subjectEndemic Disease
dc.subjectGene
dc.subjectGene Location
dc.subjectGene Mutation
dc.subjectGene Sequence
dc.subjectGenetic Association
dc.subjectGenetic Polymorphism
dc.subjectGenetic Resistance
dc.subjectGenetic Selection
dc.subjectGenetic Variability
dc.subjectHaplotype
dc.subjectHuman
dc.subjectMalaria Control
dc.subjectMdr1 Gene
dc.subjectNonhuman
dc.subjectNucleotide Sequence
dc.subjectPlasmodium Vivax
dc.subjectPlasmodium Vivax Malaria
dc.subjectPolymerase Chain Reaction
dc.subjectVacuna contra la malaria
dc.subjectCrt O génica
dc.subjectColombia
dc.subject.ddcEnfermedades 
dc.subject.lembMalaria
dc.subject.lembPlasmodium
dc.titleSimultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region
dc.typearticle
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitation(2016) World Malaria Report 2016, , WHO World Health Organization Geneva
dc.creator.googleCubides, J.R., Camargo-Ayala, P.A., Niño, C.H., Garzón-Ospina, D., Ortega-Ortegón, A., Ospina-Cantillo, E., Orduz-Durán, M.F., Patarroyo, M.E., Patarroyo, M.A.
dc.identifier.doi10.1186/s12936-018-2286-5


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