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dc.creatorObando-Martinez A.Z. 
dc.creatorCurtidor H. 
dc.creatorArévalo-Pinzón G. 
dc.creatorVanegas M. 
dc.creatorVizcaino C. 
dc.creatorPatarroyo M.A. 
dc.creatorPatarroyo M.E. 
dc.date.accessioned2020-05-25T23:56:20Z
dc.date.available2020-05-25T23:56:20Z
dc.date.created2010
dc.identifier.issn00222623
dc.identifier.issn15204804
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22401
dc.description.abstract"Detergent resistant membranes (DRMs) of Plasmodium falciparum merozoites contain a large number of glycosylphosphatidylinositol (GPI)-anchored proteins that have been implicated in interactions between merozoites and red blood cells (RBCs). In this study, two cysteine-rich proteins anchored by GPI to merozoite DRMs (Pf92 and Pf113) were studied with the aim of identifying regions actively involved in RBC invasion. By means of binding assays, high-activity binding peptides (HABPs) with a large number of binding sites per RBC were identified in Pf92 and Pf113. The nature of the RBC surface receptors for these HABPs was explored using enzyme-treated RBCs and cross-linking assays. Invasion inhibition and immunofluorescence localization studies suggest that Pf92 and Pf113 are involved in RBC invasion and that their adhesion to RBCs is mediated by such HABPs. Additionally, polymorphism and circular dichroism studies support their inclusion in further studies to design components of an antimalarial vaccine. © 2010 American Chemical Society."
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofJournal of Medicinal Chemistry, ISSN:00222623, 15204804, Vol.53, No.10 (2010); pp. 3907-3918
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77952739304&doi=10.1021%2fjm901474p&partnerID=40&md5=f25489be2eb7f9961f8752fcac0dc128
dc.sourceinstname:Universidad del Rosario
dc.sourcereponame:Repositorio Institucional EdocUR
dc.titleConserved high activity binding peptides are involved in adhesion of two detergent-resistant membrane-associated merozoite proteins to red blood cells during invasion
dc.typearticle
dc.subject.keywordBinding protein
dc.subject.keywordCell surface receptor
dc.subject.keywordgenetic
dc.subject.keywordmolecular
dc.subject.keywordsecondary
dc.subject.keywordCysteine
dc.subject.keywordDetergent
dc.subject.keywordGlycosylphosphatidylinositol
dc.subject.keywordHigh activity binding peptide
dc.subject.keywordMalaria vaccine
dc.subject.keywordPf113 protein
dc.subject.keywordPf92 protein
dc.subject.keywordProtozoal protein
dc.subject.keywordUnclassified drug
dc.subject.keywordAmino acid sequence
dc.subject.keywordAnimal experiment
dc.subject.keywordAnimal model
dc.subject.keywordArticle
dc.subject.keywordBinding affinity
dc.subject.keywordBinding assay
dc.subject.keywordBinding site
dc.subject.keywordCell adhesion
dc.subject.keywordCell invasion
dc.subject.keywordCircular dichroism
dc.subject.keywordControlled study
dc.subject.keywordCross linking
dc.subject.keywordErythrocyte
dc.subject.keywordHuman
dc.subject.keywordHuman cell
dc.subject.keywordImmunofluorescence
dc.subject.keywordLipid raft
dc.subject.keywordMerozoite
dc.subject.keywordNonhuman
dc.subject.keywordPlasmodium falciparum
dc.subject.keywordProtein binding
dc.subject.keywordProtein localization
dc.subject.keywordProtein polymorphism
dc.subject.keywordAnimals
dc.subject.keywordBinding sites
dc.subject.keywordChymotrypsin
dc.subject.keywordCircular dichroism
dc.subject.keywordCross-linking reagents
dc.subject.keywordCysteine
dc.subject.keywordDetergents
dc.subject.keywordErythrocytes
dc.subject.keywordGlycosylphosphatidylinositols
dc.subject.keywordHost-parasite interactions
dc.subject.keywordImmune sera
dc.subject.keywordMembrane proteins
dc.subject.keywordMerozoites
dc.subject.keywordModels
dc.subject.keywordNeuraminidase
dc.subject.keywordPeptides
dc.subject.keywordPlasmodium falciparum
dc.subject.keywordPolymorphism
dc.subject.keywordProtein binding
dc.subject.keywordProtein structure
dc.subject.keywordProtozoan proteins
dc.subject.keywordRabbits
dc.subject.keywordSuccinimides
dc.subject.keywordTrypsin
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doihttps://doi.org/10.1021/jm901474p
dc.relation.citationEndPage3918
dc.relation.citationIssueNo. 10
dc.relation.citationStartPage3907
dc.relation.citationTitleJournal of Medicinal Chemistry
dc.relation.citationVolumeVol. 53


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