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dc.creatorRamos, Andrea Estefanía 
dc.creatorMuñoz, Marina 
dc.creatorCortés-Vecino, Jesús Alfredo 
dc.creatorBarato, Paola 
dc.creatorPatarroyo, Manuel A. 
dc.date.accessioned2020-05-25T23:57:49Z
dc.date.available2020-05-25T23:57:49Z
dc.date.created2017
dc.identifier.issn17563305
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22749
dc.description.abstract"Background: Neospora caninum is a cyst-forming, coccidian parasite which is known to cause neurological disorders in dogs and abortion and neonatal mortality in cows and other livestock. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay based on the Neospora caninum Nc-5 gene and compares its efficacy for detecting DNA to that of a semi-nested PCR test. Results: Six primers were designed based on the Nc-5 repeat region of N. caninum. Specific LAMP primers led to successful amplification of N. caninum DNA at 63 °C in 30 min. The LAMP assay was highly specific (i.e. it did not reveal cross-reactivity with other parasite species) and had a low N. caninum plasmid DNA limit of detection (1 fg), which is ten times higher than that for the semi-nested PCR. LAMP applicability was evaluated using a set of naturally-infected samples (59 from canine faeces and five from bovine abortions). Thirty-nine percent (25/64) of the naturally-infected samples were positive for N. caninum DNA by LAMP and 36% (23/64) by semi-nested PCR. However, the LAMP assay is much faster to perform than semi-nested PCR and provides results in 30 min. Conclusion: The optimized reaction conditions described in this study resulted in a sensitive, specific and rapid technique for detecting N. caninum DNA. Considering the advantages of LAMP for detecting N. caninum DNA, further assays aimed at testing its usefulness on a wider range of field samples are recommended. © 2017 The Author(s)."
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofParasites and Vectors, ISSN:17563305, Vol.10, No.1 (2017)
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85036646650&doi=10.1186%2fs13071-017-2549-y&partnerID=40&md5=9c08ad6642204e4759baabc4403b3906
dc.sourceinstname:Universidad del Rosario
dc.sourcereponame:Repositorio Institucional EdocUR
dc.titleA novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA
dc.typearticle
dc.publisherBioMed Central Ltd.
dc.subject.keywordDna
dc.subject.keywordprotozoan
dc.subject.keywordParasite antigen
dc.subject.keywordprotozoan
dc.subject.keywordPrimer dna
dc.subject.keywordProtozoal dna
dc.subject.keywordArticle
dc.subject.keywordControlled study
dc.subject.keywordCryptosporidium parvum
dc.subject.keywordDna determination
dc.subject.keywordDna sequence
dc.subject.keywordGene
dc.subject.keywordGene amplification
dc.subject.keywordHammondia hammondi
dc.subject.keywordLimit of detection
dc.subject.keywordLoop mediated isothermal amplification
dc.subject.keywordNc 5 gene
dc.subject.keywordNeospora caninum
dc.subject.keywordNeosporosis
dc.subject.keywordNonhuman
dc.subject.keywordPolymerase chain reaction
dc.subject.keywordProtozoon
dc.subject.keywordSarcocystis
dc.subject.keywordSarcocystis cruzi
dc.subject.keywordSarcocystis hominis
dc.subject.keywordSequence alignment
dc.subject.keywordToxoplasma gondii
dc.subject.keywordAnimal
dc.subject.keywordBovine
dc.subject.keywordCattle disease
dc.subject.keywordCoccidiosis
dc.subject.keywordDog
dc.subject.keywordDog disease
dc.subject.keywordFeces
dc.subject.keywordFemale
dc.subject.keywordGenetics
dc.subject.keywordIsolation and purification
dc.subject.keywordNeospora
dc.subject.keywordNucleic acid amplification
dc.subject.keywordParasitology
dc.subject.keywordPregnancy
dc.subject.keywordProcedures
dc.subject.keywordSensitivity and specificity
dc.subject.keywordTemperature
dc.subject.keywordVeterinary
dc.subject.keywordAnimals
dc.subject.keywordAntigens
dc.subject.keywordCattle
dc.subject.keywordCattle diseases
dc.subject.keywordCoccidiosis
dc.subject.keywordDna primers
dc.subject.keywordDna
dc.subject.keywordDog diseases
dc.subject.keywordDogs
dc.subject.keywordFeces
dc.subject.keywordFemale
dc.subject.keywordLimit of detection
dc.subject.keywordNeospora
dc.subject.keywordNucleic acid amplification techniques
dc.subject.keywordPolymerase chain reaction
dc.subject.keywordPregnancy
dc.subject.keywordSensitivity and specificity
dc.subject.keywordTemperature
dc.subject.keywordLoop-mediated isothermal amplification
dc.subject.keywordNc-5 gene
dc.subject.keywordNeospora caninum
dc.subject.keywordNeosporosis
dc.subject.keywordSemi-nested pcr
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doihttps://doi.org/10.1186/s13071-017-2549-y
dc.relation.citationIssueNo. 1
dc.relation.citationTitleParasites and Vectors
dc.relation.citationVolumeVol. 10


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