Passive transfer of Plasmodium falciparum MSP-2 pseudopeptide-induced antibodies efficiently controlled parasitemia in Plasmodium berghei-infected mice
AuthorMartínez, Paola A.
Lesmes, Liliana P.
Patarroyo, Manuel Elkin
Lozano, José Manuel
We have developed monoclonal antibodies directed against the pseudopeptide ?-130, derived from the highly conserved malarial antigen Plasmodium falciparum merozoite surface protein 2 (MSP-2), for obtaining novel molecular tools with potential applications in the control of malaria. Following isotype switching, these antibodies were tested for their ability to suppress blood-stage parasitemia through passive immunization in malaria-infected mice. Some proved totally effective in suppressing a lethal blood-stage challenge infection and others reduced malarial parasitemia. Protection against P. berghei malaria following Ig passive immunization can be associated with specific immunoglobulins induced by a site-directed designed MSP-2 reduced amide pseudopeptide. © 2008 Elsevier Inc. All rights reserved.
Immunoglobulin ; protozoan ; Immunoglobulin class ; Malaria vaccine ; Merozoite surface protein 2 ; Monoclonal antibody ; Monoclonal antibody anti pseudopeptide psi 130 ; Pseudopeptide ; Unclassified drug ; Animal cell ; Animal experiment ; Animal model ; Animal tissue ; Article ; Controlled study ; Drug design ; Drug mechanism ; Drug synthesis ; Female ; Mouse ; Nonhuman ; Parasitemia ; Passive immunization ; Plasmodium berghei infection ; Plasmodium falciparum ; Priority journal ; Animals ; Antibodies, protozoan ; Antigens, protozoan ; Disease models, animal ; Female ; Immunization, passive ; Malaria ; Mice ; Mice, inbred balb c ; Parasitemia ; Plasmodium berghei ; Plasmodium falciparum ; Protozoan proteins ; Recombinant proteins ; Murinae ; Mus ; Plasmodium berghei ; Plasmodium falciparum ; Anti-malarial vaccine ; Ig isotype ; In vivo neutralizing activity ; Murine malarial infection ; Pseudopeptide ;
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