Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

Rojas-Caraballo, Jose; Mongui, Alvaro; Giraldo, Manuel A.; Delgado, Gabriela; Granados, Diana; Millan-Cortes, Diana; Martinez, Paola; Rodriguez, Raul; Patarroyo, Manuel A.

doi:https://doi.org/10.1016/j.vaccine.2009.02.083

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Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

https://repository.urosario.edu.co/handle/10336/23450
https://doi.org/10.1016/j.vaccine.2009.02.083

Autores

Rojas-Caraballo, Jose

Mongui, Alvaro

Giraldo, Manuel A.

Delgado, Gabriela

Granados, Diana

Millan-Cortes, Diana

Martinez, Paola

Rodriguez, Raul

Patarroyo, Manuel A.

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2009

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Abstract

Rhoptry proteins have been extensively shown to be important in invasion and parasitophorous vacuole (PV) formation. This work evaluates the immunogenicity and protective efficacy of Plasmodium vivax RAP2 in the non-human Aotus primate model, when expressed as a recombinant molecule in E. coli and formulated in Freund and Alum hydroxide adjuvants. Our results show that rPvRAP2 is immunogenic in both formulations, finding a trend of higher cytokine levels in immunized monkeys, specially in IL-4 levels (using Freund's adjuvant) and IL-5 (using Alum hydroxide). RAP2 is suggested as a P. vivax-vaccine candidate since immunized monkeys exhibited lower parasitemias than control groups after being experimentally challenged with the P. vivax VCG-I strain. © 2009 Elsevier Ltd. All rights reserved.

Keywords

Alum hydroxide , Antibody , Clindamycin , Cytokine , Freund adjuvant , Gamma interferon , Immunological adjuvant , Interleukin 2 , Interleukin 4 , Interleukin 5 , Interleukin 6 , Malaria vaccine , Primaquine , Pyrimethamine , Quinine sulfate , Recombinant protein pvrap2 vaccine , Recombinant rhoptry associated protein 2 vaccine , Sulfadoxine , Tumor necrosis factor , Unclassified drug , Animal experiment , Animal model , Antibody response , Antibody specificity , Antibody titer , Aotus nancymaae , Article , Cell vacuole , Cellular immunity , Controlled study , Cytokine production , Drug efficacy , Enzyme linked immunosorbent assay , Escherichia coli , Humoral immunity , Immune response , Immunization , Immunofluorescence , Immunogenicity , Monkey , Nonhuman , Parasitemia , Plasmodium vivax , Priority journal , Protection , Protein expression , Western blotting , Animals , Aotidae , Disease models , Immunogenetic processes , Malaria vaccines , Plasmodium vivax , Protozoan proteins , Immune response , Plasmodium vivax , Rhoptry-associated protein , Vaccine candidate