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dc.creatorBlack, Allison 
dc.creatorMoncla, Louise H. 
dc.creatorLaiton-Donato, Katherine 
dc.creatorPotter, Barney 
dc.creatorPardo, Lissethe 
dc.creatorRico, Angelica 
dc.creatorTovar, Catalina 
dc.creatorRojas, Diana P. 
dc.creatorLongini, Ira M. 
dc.creatorHalloran, M. Elizabeth 
dc.creatorPeláez-Carvajal, Dioselina 
dc.creatorRamírez, Juan David 
dc.creatorMercado-Reyes, Marcela 
dc.creatorBedford, Trevor 
dc.date.accessioned2020-05-26T00:03:12Z
dc.date.available2020-05-26T00:03:12Z
dc.date.created2019
dc.identifier.issn14712334
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23569
dc.description.abstract"BACKGROUND: Colombia was the second most affected country during the American Zika virus (ZIKV) epidemic, with over 109,000 reported cases. Despite the scale of the outbreak, limited genomic sequence data were available from Colombia. We sought to sequence additional samples and use genomic epidemiology to describe ZIKV dynamics in Colombia. METHODS: We sequenced ZIKV genomes directly from clinical diagnostic specimens and infected Aedes aegypti samples selected to cover the temporal and geographic breadth of the Colombian outbreak. We performed phylogeographic analysis of these genomes, along with other publicly-available ZIKV genomes from the Americas, to estimate the frequency and timing of ZIKV introductions to Colombia. RESULTS: We attempted PCR amplification on 184 samples; 19 samples amplified sufficiently to perform sequencing. Of these, 8 samples yielded sequences with at least 50% coverage. Our phylogeographic reconstruction indicates two separate introductions of ZIKV to Colombia, one of which was previously unrecognized. We find that ZIKV was first introduced to Colombia in February 2015 (95%CI: Jan 2015 - Apr 2015), corresponding to 5 to 8 months of cryptic ZIKV transmission prior to confirmation in September 2015. Despite the presence of multiple introductions, we find that the majority of Colombian ZIKV diversity descends from a single introduction. We find evidence for movement of ZIKV from Colombia into bordering countries, including Peru, Ecuador, Panama, and Venezuela. CONCLUSIONS: Similarly to genomic epidemiological studies of ZIKV dynamics in other countries, we find that ZIKV circulated cryptically in Colombia. More accurately dating when ZIKV was circulating refines our definition of the population at risk. Additionally, our finding that the majority of ZIKV transmission within Colombia was attributable to transmission between individuals, rather than repeated travel-related importations, indicates that improved detection and control might have succeeded in limiting the scale of the outbreak within Colombia."
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofBMC infectious diseases, ISSN:14712334, Vol.19, No.1 (2019); pp. 963-
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85074950413&doi=10.1186%2fs12879-019-4566-2&partnerID=40&md5=1c2313c2a7acf5e7ffb165b5544bf988
dc.sourceinstname:Universidad del Rosario
dc.sourcereponame:Repositorio Institucional EdocUR
dc.titleGenomic epidemiology supports multiple introductions and cryptic transmission of Zika virus in Colombia
dc.typearticle
dc.publisherNLM (Medline)
dc.subject.keywordAedes
dc.subject.keywordAnimal
dc.subject.keywordViral
dc.subject.keywordMolecular
dc.subject.keywordClassification
dc.subject.keywordColombia
dc.subject.keywordEpidemic
dc.subject.keywordGenetic variation
dc.subject.keywordGenetics
dc.subject.keywordHuman
dc.subject.keywordIsolation and purification
dc.subject.keywordMolecular evolution
dc.subject.keywordPhylogeny
dc.subject.keywordPhylogeography
dc.subject.keywordVirology
dc.subject.keywordVirus genome
dc.subject.keywordZika fever
dc.subject.keywordZika virus
dc.subject.keywordAedes
dc.subject.keywordAnimals
dc.subject.keywordColombia
dc.subject.keywordDisease Outbreaks
dc.subject.keywordEvolution
dc.subject.keywordGenetic Variation
dc.subject.keywordGenome
dc.subject.keywordHumans
dc.subject.keywordPhylogeny
dc.subject.keywordPhylogeography
dc.subject.keywordZika Virus
dc.subject.keywordZika Virus Infection
dc.subject.keywordColombia
dc.subject.keywordGenomic epidemiology
dc.subject.keywordPhylogeography
dc.subject.keywordVirus evolution
dc.subject.keywordZika virus
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doihttps://doi.org/10.1186/s12879-019-4566-2
dc.relation.citationEndPage
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage963
dc.relation.citationTitleBMC infectious diseases
dc.relation.citationVolumeVol. 19


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