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Agreement of the Kato-Katz test established by the WHO with samples fixed with sodium acetate analyzed at 6 months to diagnose intestinal geohelminthes
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Autores
Fernández-Niño, Julián Alfredo
Ramírez, Juan David
López, Myriam Consuelo
Moncada, Ligia Inés
Reyes, Patricia
Heredia, Rubén Darío
Fecha
2015
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Elsevier
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Abstract
The aim of this study was to evaluate the performance of the Kato-Katz test (WHO version) with stool samples from a rural area, fixed with sodium acetate (SAF). The Kato-Katz test was used to compare unfixed samples (conventional test) with the same samples containing SAF fixative at time 0 and at 6 months. The study included stools from 154 subjects.A marginally statistically significant decrease in prevalence was estimated only for hookworm, when comparing unfixed samples versus the SAF fixed samples read at 6 months (. p=. 0.06). A significant reduction in parasite load was found for hookworm (. p less than . 0.01) and Trichuris trichiura (. p less than . 0.01) between the unfixed and the fixed sample read at 6 months, but not for Ascaris lumbricoides (. p=. 0.10). This research suggests that the SAF fixative solution is a good option for transporting samples for diagnosis, especially in rural areas in developing countries. © 2015 Elsevier B.V.
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Keywords
Acetic acid , parasitic , Fixative , parasitic , Acetic acid , Soil , Acetate , Developing world , Endoparasite , Fecal pellet , Public health , Sodium , Soilborne disease , World health organization , Article , Ascaris lumbricoides , Controlled study , Diagnostic test accuracy study , Feces analysis , Helminthiasis , Hookworm , Intestinal geohelminthes , Intestine infection , Kato katz test , Microbiological examination , Nonhuman , Parasite identification , Parasite load , Prevalence , Rural area , Trichuris trichiura , World health organization , Adult , Animal , Ascariasis , Colombia , Comparative study , Developing country , Feces , Female , Helminthiasis , Hookworm infections , Human , Intestinal diseases , Intestine , Isolation and purification , Male , Middle aged , Parasite identification , Parasitology , Sensitivity and specificity , Soil , Time , Transport at the cellular level , Trichuris , Colombia , Ancylostomatoidea , Ascaris , Ascaris lumbricoides , Trichuris trichiura , Vermes , Adult , Ancylostomatoidea , Animals , Ascariasis , Ascaris lumbricoides , Biological transport , Colombia , Developing countries , Feces , Female , Fixatives , Helminthiasis , Hookworm infections , Humans , Intestinal diseases , Intestines , Male , Middle aged , Parasite egg count , Parasite load , Prevalence , Sensitivity and specificity , Sodium acetate , Soil , Time factors , Trichuris , World health organization , Ascaris , Colombia , Diagnosis , Hookworm , Soil-transmitted helminth
