Agreement of the Kato-Katz test established by the WHO with samples fixed with sodium acetate analyzed at 6 months to diagnose intestinal geohelminthes
Date
2015Author
Fernández-Niño, Julián AlfredoRamírez, Juan David
López, Myriam Consuelo
Moncada, Ligia Inés
Reyes, Patricia
Heredia, Rubén Darío
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Citation
URI
https://doi.org/10.1016/j.actatropica.2015.03.004https://repository.urosario.edu.co/handle/10336/23619
Abstract
The aim of this study was to evaluate the performance of the Kato-Katz test (WHO version) with stool samples from a rural area, fixed with sodium acetate (SAF). The Kato-Katz test was used to compare unfixed samples (conventional test) with the same samples containing SAF fixative at time 0 and at 6 months. The study included stools from 154 subjects.A marginally statistically significant decrease in prevalence was estimated only for hookworm, when comparing unfixed samples versus the SAF fixed samples read at 6 months (. p=. 0.06). A significant reduction in parasite load was found for hookworm (. p less than . 0.01) and Trichuris trichiura (. p less than . 0.01) between the unfixed and the fixed sample read at 6 months, but not for Ascaris lumbricoides (. p=. 0.10). This research suggests that the SAF fixative solution is a good option for transporting samples for diagnosis, especially in rural areas in developing countries. © 2015 Elsevier B.V.
Keyword
Acetic acid ; parasitic ; Fixative ; parasitic ; Acetic acid ; Soil ; Acetate ; Developing world ; Endoparasite ; Fecal pellet ; Public health ; Sodium ; Soilborne disease ; World health organization ; Article ; Ascaris lumbricoides ; Controlled study ; Diagnostic test accuracy study ; Feces analysis ; Helminthiasis ; Hookworm ; Intestinal geohelminthes ; Intestine infection ; Kato katz test ; Microbiological examination ; Nonhuman ; Parasite identification ; Parasite load ; Prevalence ; Rural area ; Trichuris trichiura ; World health organization ; Adult ; Animal ; Ascariasis ; Colombia ; Comparative study ; Developing country ; Feces ; Female ; Helminthiasis ; Hookworm infections ; Human ; Intestinal diseases ; Intestine ; Isolation and purification ; Male ; Middle aged ; Parasite identification ; Parasitology ; Sensitivity and specificity ; Soil ; Time ; Transport at the cellular level ; Trichuris ; Colombia ; Ancylostomatoidea ; Ascaris ; Ascaris lumbricoides ; Trichuris trichiura ; Vermes ; Adult ; Ancylostomatoidea ; Animals ; Ascariasis ; Ascaris lumbricoides ; Biological transport ; Colombia ; Developing countries ; Feces ; Female ; Fixatives ; Helminthiasis ; Hookworm infections ; Humans ; Intestinal diseases ; Intestines ; Male ; Middle aged ; Parasite egg count ; Parasite load ; Prevalence ; Sensitivity and specificity ; Sodium acetate ; Soil ; Time factors ; Trichuris ; World health organization ; Ascaris ; Colombia ; Diagnosis ; Hookworm ; Soil-transmitted helminth ;
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