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Polymerase chain reaction and molecular diagnostics
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Autores
Villegas Gálvez, Victoria Eugenia
Sánchez M.C.
Chuaire L.
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Fecha
2009
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Abstract
PCR, acronym of polymerase chain reaction, has revolutionized the field of molecular diagnostics to the point that it currently represents the fastest-growing segment in clinical laboratories worldwide, where it has become an invaluable tool. This paper describes the fundamentals of PCR and its developments in some of its many applications in the diagnosis, from its beginnings to the present. © 2009 Universidad del Valle, Facultad de Salud.
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Keywords
BRCA1 protein , Uvomorulin , CD4+ T lymphocyte , Diagnostic value , Hepatitis C , Human immunodeficiency virus infection , Laboratory diagnosis , Microbial identification , Mutational analysis , Papanicolaou test , Polymerase chain reaction , Quantitative analysis , Review , Variable number of tandem repeat , History , Methods , PCR , Utilization
