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Analysis of DC-SIGN (CD209) Functional Variants in Patients with Tuberculosis

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Gómez L.M.
Anaya, Juan-Manuel
Sierra-Filardi E.
Cadena J.
Corbí Á.
Martín J.

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2006

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Abstract
Several lines of evidence suggest that host genetic factors controlling the immune response influence infection by Mycobacterium tuberculosis. Recently, DC-SIGN has been shown to be the major M. tuberculosis receptor on dendritic cells (DCs). The aim of this study was to investigate the influence of DC-SIGN functional polymorphisms -336G/A SNP in the promoter region and insertion/deletion in the 'neck' region on the predisposition to tuberculosis. We performed an association study in 110 HIV-negative tuberculosis patients and 299 matched controls. In addition, a total of 155 healthy controls were screened for the tuberculin skin test (TST). DC-SIGN -336 SNP detection was performed by the real-time polymerase chain reaction technology, using the TaqMan 5? allele. The insertion/deletion in the 'neck' region was analyzed by polymerase chain reaction with specific primers. Although an increased frequency of the G allele in tuberculosis patients (23%), as compared with controls (19%), was observed, differences were not statistically significant (OR = 1.31, 95% CI = 0.89-1.94, P = 0.14). On the other hand, DC-SIGN repeat polymorphism in the 'neck' region had a very low frequency in the analyzed population. We conclude that the studied polymorphisms are not relevant risk factors for developing tuberculosis in Northwestern Colombian individuals. © 2006 American Society for Histocompatibility and Immunogenetics.
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CD209 antigen , Adult , Cell Surface , Single Nucleotide , Pulmonary , Delayed , C-Type , Allele , Article , Colombia , Controlled study , Dendritic cell , Female , Gene deletion , Gene insertion , Genetic polymorphism , Genetic predisposition , Human , Human immunodeficiency virus , Major clinical study , Male , Mycobacterium tuberculosis , Neck , Nonhuman , Nucleotide sequence , Priority journal , Promoter region , Real time polymerase chain reaction , Risk factor , Single nucleotide polymorphism , Statistical significance , Tuberculin test , Tuberculosis , Adult , Cell Adhesion Molecules , Colombia , Female , Gene Frequency , Genotype , Heterozygote , Homozygote , Humans , Hypersensitivity , Lectins , Male , Middle Aged , Polymorphism , Receptors , Tuberculosis , Tuberculosis , CD209 , Colombia , DC-SIGN , Tuberculin skin test , Tuberculosis
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