Ítem
Acceso Abierto
The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region
Título de la revista
Autores
Villa, Lina Marcela
Guhl, Felipe
Zabala, Daniel
Ramírez, Juan David
Urrea, Daniel Alfonso
Hernández, Diana Carolina
Cucunubá, Zulma
Montilla, Marleny
Carranza, Julio César
Rueda, Karina
Archivos
Fecha
2013-11
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Editor
Instituto Oswaldo Cruz
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Resumen
Abstract
A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruzi I to distinguish TcIDOMfrom sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.
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Keywords
Spliced-leader intergenic region , Trypanosoma cruzi I , PCR , Chagas disease , Domestic cycle , Sylvatic cycle
