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Human papillomavirus detection from human immunodeficiency virus-infected colombian women's paired urine and cervical samples

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Date
2013-02-13
Author
Munoz, Marina
Camargo, Milena
Soto De Leon, Sara C
Sánchez, Ricardo
Parra, Diana
Pineda, Andrea C.
Sussmann, Otto
Perez-Prados, Antonio
Patarroyo, Manuel E.
Patarroyo, Manuel A.Autoridad Universidad de Rosario
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Citation
URI

http://repository.urosario.edu.co/handle/10336/8781

Abstract
Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance.

Keyword

POSITIVE WOMEN ; HPV INFECTION ; DNA DETECTION ; PRIMER SETS ; PCR ; CANCER ;

Subject

Ginecología & otras especialidades médicas ;

Source link

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0056509...

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