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Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis: differential delivery of healing biomarkers

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dc.creatorFontanilla M.R.spa
dc.creatorCasadiegos S.spa
dc.creatorBustos R.H.spa
dc.creatorPatarroyo M.A.spa
dc.date.accessioned2020-05-26T00:06:13Z
dc.date.available2020-05-26T00:06:13Z
dc.date.created2018spa
dc.description.abstractCytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario provided by clinical follow-up data and histological and histomorphometric analyses. This work assessed and compared the healing of full-thickness skin wounds grafted with artificial dermis made with autologous skin fibroblasts and unidirectional or multidirectional type I collagen scaffolds to test this hypothesis. Biomarkers of healing were detected and quantified in the culture medium of artificial dermis and exudates from the grafted wounds. Clinical follow-up of animals and histological and histomorphometric analysis showed differences in graft integration, wound closure, and histological and histomorphometric parameters. Surface plasmon resonance quantification of 13 healing biomarkers indicated differential secretion of most of the quantified factors in culture medium by the multidirectional and unidirectional artificial dermis. Also, there were significant differences between the concentration of some of the factors analyzed in the exudates of wounds grafted with the evaluated artificial dermis. These findings suggest that differential delivery of healing biomarkers induced by the directionality of the scaffold used to produce the multidirectional and unidirectional dermis was sufficient to create two skin wound microenvironments that determined a different outcome of healing. Overall, data indicate that healing of wounds grafted with multidirectional autologous artificial dermis is better than that of the wounds grafted with the unidirectional one. © 2018, Controlled Release Society.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s13346-018-0528-2
dc.identifier.issn21903948
dc.identifier.issn2190393X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23868
dc.language.isoengspa
dc.publisherSpringer Verlagspa
dc.relation.citationEndPage1024
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage1014
dc.relation.citationTitleDrug Delivery and Translational Research
dc.relation.citationVolumeVol. 8
dc.relation.ispartofDrug Delivery and Translational Research, ISSN:21903948, 2190393X, Vol.8, No.5 (2018); pp. 1014-1024spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85052314960&doi=10.1007%2fs13346-018-0528-2&partnerID=40&md5=30520103235de831c9c2ecacfa2270ddspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBiological markerspa
dc.subject.keywordanimaleng
dc.subject.keywordBiological markerspa
dc.subject.keywordChemokinespa
dc.subject.keywordCytokinespa
dc.subject.keywordSignal peptidespa
dc.subject.keywordAnimal cellspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAnimal modelspa
dc.subject.keywordAnimal tissuespa
dc.subject.keywordArticlespa
dc.subject.keywordComparative studyspa
dc.subject.keywordControlled studyspa
dc.subject.keywordFollow upspa
dc.subject.keywordGranulation tissuespa
dc.subject.keywordHistopathologyspa
dc.subject.keywordLagomorphspa
dc.subject.keywordMorphometryspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPriority journalspa
dc.subject.keywordSkin graftspa
dc.subject.keywordSkin injuryspa
dc.subject.keywordSurface plasmon resonancespa
dc.subject.keywordWound closurespa
dc.subject.keywordWound fluidspa
dc.subject.keywordWound healingspa
dc.subject.keywordAnimalspa
dc.subject.keywordArtificial skinspa
dc.subject.keywordAutograftspa
dc.subject.keywordDisease modelspa
dc.subject.keywordEpidermisspa
dc.subject.keywordMetabolismspa
dc.subject.keywordSkin diseasespa
dc.subject.keywordSkin transplantationspa
dc.subject.keywordTissue scaffoldspa
dc.subject.keywordTransplantationspa
dc.subject.keywordAnimalsspa
dc.subject.keywordAutograftsspa
dc.subject.keywordBiomarkersspa
dc.subject.keywordChemokinesspa
dc.subject.keywordCollagen type ispa
dc.subject.keywordCytokinesspa
dc.subject.keywordDisease modelseng
dc.subject.keywordEpidermisspa
dc.subject.keywordIntercellular signaling peptides and proteinsspa
dc.subject.keywordSkin diseasesspa
dc.subject.keywordSkin transplantationspa
dc.subject.keywordSkineng
dc.subject.keywordSurface plasmon resonancespa
dc.subject.keywordTissue scaffoldsspa
dc.subject.keywordWound healingspa
dc.subject.keywordDifferential delivery of bioactive compoundsspa
dc.subject.keywordFiber orientationspa
dc.subject.keywordHealing biomarkersspa
dc.subject.keywordHealing of full-thickness skin woundsspa
dc.subject.keywordMultidirectional and unidirectional autologous artificial dermisspa
dc.subject.keywordSpr quantificationspa
dc.titleComparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis: differential delivery of healing biomarkersspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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