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Reticulocyte count in red-blood-cell units stored in AS-1

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dc.creatorUrbina Bonilla, Adriana del Pilarspa
dc.creatorPalomino, F.spa
dc.date.accessioned2020-05-26T00:02:24Z
dc.date.available2020-05-26T00:02:24Z
dc.date.created2013spa
dc.description.abstractBackground and objectives: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. Materials and methods: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. Results: Reticulocyte count was 26·8 ± 4·6 × 109/l at week 0·5 and 8·2 ±2·9 × 109/l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 109/l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 109/l at week 0·5 and decreased by weeks 4 and 6. Conclusion: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. © 2013 International Society of Blood Transfusion.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/vox.12011
dc.identifier.issn14230410
dc.identifier.issn00429007
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23483
dc.language.isoengspa
dc.relation.citationEndPage336
dc.relation.citationIssueNo. 4
dc.relation.citationStartPage331
dc.relation.citationTitleVox Sanguinis
dc.relation.citationVolumeVol. 104
dc.relation.ispartofVox Sanguinis, ISSN:14230410, 00429007, Vol.104, No.4 (2013); pp. 331-336spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84876728529&doi=10.1111%2fvox.12011&partnerID=40&md5=ae20a760349d573f4a2f552851f86a01spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordThiazole orangespa
dc.subject.keywordArticlespa
dc.subject.keywordErythrocyte concentratespa
dc.subject.keywordErythrocyte preservationspa
dc.subject.keywordFlow cytometryspa
dc.subject.keywordFluorescence analysisspa
dc.subject.keywordHemolysisspa
dc.subject.keywordPriority journalspa
dc.subject.keywordReticulocyte countspa
dc.subject.keywordBlood banksspa
dc.subject.keywordBlood preservationspa
dc.subject.keywordErythrocytesspa
dc.subject.keywordFlow cytometryspa
dc.subject.keywordHumansspa
dc.subject.keywordReticulocyte countspa
dc.subject.keywordReticulocytesspa
dc.subject.keywordBlood bankspa
dc.subject.keywordHigh-fluorescence reticulocytesspa
dc.subject.keywordRed-blood-cell unitspa
dc.subject.keywordReticulocytespa
dc.titleReticulocyte count in red-blood-cell units stored in AS-1spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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