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Proteolytic Processing of the Cyt1Ab1 Toxin Produced by Bacillus thuringiensis Subsp. Medellin.

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dc.creatorVanegas Murcia, Magnoliaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.creatorEscibar, Elizabethspa
dc.creatorSegura, Cesarspa
dc.creatorOrduz, Sergiospa
dc.date.accessioned2020-08-19T14:40:18Z
dc.date.available2020-08-19T14:40:18Z
dc.date.created2000spa
dc.description.abstractBacillus thuringiensis produces d-endotoxins that require proteolytic processing to become active. The activation of the B. thuringiensis subsp. medellin 28 kDa (Cyt1Ab1) cytolytic toxin by trypsin, chymotrypsin and gut extract from Culex quinquefasciatus larvae was analyzed. The Cyt1Ab1 toxin of B. thuringiensis subsp. medellin was processed by all proteases tested to fragments between 23 and 25 kDa, while processing of the Cyt1Aa1 toxin produce fragments between 22.5 and 24.5 kDa. The Cyt1Ab1 toxin was preferentially processed at the alkaline pH of 12. The in vitro proteolytic processing of the Cyt1Ab1 toxin by C. quinquefasciatus larvae midgut extract showed a 25 kDa fragment; a similar result was observed when the activation was performed in the in vivo experiments. The solubilized Cyt1Ab1 toxin and the protease resistant cores generated by in vitro processing showed hemolytic activity but not mosquitocidal activity. Amino terminal sequence of the C. quinquefasciatus gut extract resistant fragment indicated that the cutting site was located between Lys31 and Asp32, with a sequence DDPNEKNNHNS; while for the trypsin-resistant fragment the cutting site was determined between Leu29 and Arg30, and for the chymotrypsin-resistant fragment between Arg30 and Lys3eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttp://dx.doi.org/10.1590/S0074-02762000000500014
dc.identifier.issnISSN: 0074-0276
dc.identifier.issnEISSN: 1678-8060
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26816
dc.language.isoengspa
dc.publisherFundação Oswaldo Cruzspa
dc.relation.citationEndPage700
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage693
dc.relation.citationTitleMemorias do Instituto Oswaldo Cruz
dc.relation.citationVolumeVol. 95
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz, ISSN: 0074-0276; EISSN: 1678-8060, Vol.95, No.5 (2000); pp. 693-700spa
dc.relation.urihttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000500014spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceMemorias do Instituto Oswaldo Cruzspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordBacillus thuringiensisspa
dc.subject.keywordCyt1Ab1spa
dc.subject.keywordCytolyticspa
dc.subject.keywordHemolyticspa
dc.subject.keywordProteolytic processingspa
dc.subject.keywordMidgut proteasesspa
dc.titleProteolytic Processing of the Cyt1Ab1 Toxin Produced by Bacillus thuringiensis Subsp. Medellin.spa
dc.title.TranslatedTitleProcesamiento proteolítico de la toxina Cyt1Ab1 producida por Bacillus thuringiensis Subsp. Medellín.spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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