2026-04-26T20:56:16Zhttps://repository.urosario.edu.co/oai/requestoai:repository.urosario.edu.co:10336/221262022-05-02T07:37:18Zcom_10336_39998com_10336_563col_10336_683
Ramírez, Juan David
Méndez-Sarmiento, Claudia
Herrera, Giovanny
Muskus, Carlos
Duque, María Clara
Butcher, Robert
2020-05-25T23:55:33Z
2020-05-25T23:55:33Z
2019
Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform. © 2018 The Author(s)
application/pdf
https://doi.org/10.1016/j.ijid.2018.10.029
12019712
https://repository.urosario.edu.co/handle/10336/22126
eng
Elsevier B.V.
3
1
International Journal of Infectious Diseases
Vol. 79
International Journal of Infectious Diseases, ISSN:12019712, Vol.79,(2019); pp. 1-3
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057876716&doi=10.1016%2fj.ijid.2018.10.029&partnerID=40&md5=df03529c552cdaa5301dfc4023ee8107
info:eu-repo/semantics/openAccess
Abierto (Texto Completo)
instname:Universidad del Rosario
reponame:Repositorio Institucional EdocUR
Protozoal DNA
Protozoal DNA
Article
Droplet digital polymerase chain reaction
Human
Leishmania
Leishmania braziliensis
Leishmania donovani
Leishmania guyanensis
Leishmania infantum
Leishmania major
Leishmania mexicana
Leishmania panamensis
Limit of detection
Microorganism detection
Molecular pathology
Skin leishmaniasis
Task performance
Validation study
Evaluation study
Female
Genetics
Isolation and purification
Leishmania
Parasitology
Procedures
Real time polymerase chain reaction
Sensitivity and specificity
Skin leishmaniasis
Female
Humans
Leishmania
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Cutaneous Leishmaniasis
Ddpcr
Leishmania
Molecular diagnosis
Cutaneous
Protozoan
DNA
Leishmaniasis
Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients
article
info:eu-repo/semantics/publishedVersion
Artículo