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Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
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Etienne, Kizee A.
Roe, Chandler C.
Smith, Rachel M.
Vallabhaneni, Snigdha
Duarte-Rey, Carolina
Escandón, Patricia
Castañeda, Elizabeth
Gómez, Beatriz L.
Bedout, Catalina de
López, Luisa F.
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2016-03
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Abstract
We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved.
Palabras clave
Infección del torrente sanguíneo , Artículo clínico , Estudio controlado , Secuencia génica , Análisis del genoma humano , Filogenia Infección del torrente sanguíneo de Sarocladium kiliense , Polimorfismo de nucleótido simple
Keywords
Bloodstream infection , Clinical article , Controlled study , Gene sequence , Genome analysis , Phylogeny , Sarocladium kiliense bloodstream infection , Single nucleotide polymorphism
