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Immunization with hepatitis B vaccine accelerates SLE-like disease in a murine model
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Agmon-Levin, Nancy
Arango, María-Teresa
Kivity, Shaye
Katzav, Aviva
Gilburd, Boris
Blank, Miri
Tomer, Nir
Volkov, Alex
Barshack, Iris
Chapman, Joab
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2014
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Academic Press
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Abstract
Hepatitis-B vaccine (HBVv) can prevent HBV-infection and associated liver diseases. However, concerns regarding its safety, particularly among patients with autoimmune diseases (i.e. SLE) were raised. Moreover, the aluminum adjuvant in HBVv was related to immune mediated adverse events. Therefore, we examined the effects of immunization with HBVv or alum on SLE-like disease in a murine model.NZBWF1 mice were immunized with HBVv (Engerix), or aluminum hydroxide (alum) or phosphate buffered saline (PBS) at 8 and 12 weeks of age. Mice were followed for weight, autoantibodies titers, blood counts, proteinuria, kidney histology, neurocognitive functions (novel object recognition, staircase, Y-maze and the forced swimming tests) and brain histology.Immunization with HBVv induced acceleration of kidney disease manifested by high anti-dsDNA antibodies (. p less than 0.01), early onset of proteinuria (. p less than 0.05), histological damage and deposition of HBs antigen in the kidney. Mice immunized with HBVv and/or alum had decreased cells counts mainly of the red cell lineage (. p less than 0.001), memory deficits (. p less than 0.01), and increased activated microglia in different areas of the brain compare with mice immunized with PBS. Anxiety-like behavior was more pronounced among mice immunized with alum.In conclusion, herein we report that immunization with the HBVv aggravated kidney disease in an animal model of SLE. Immunization with either HBVv or alum affected blood counts, neurocognitive functions and brain gliosis. Our data support the concept that different component of vaccines may be linked with immune and autoimmune mediated adverse events. © 2014 Elsevier Ltd.
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Keywords
Aluminum hydroxide , animal , Hepatitis b surface antigen , Immunological adjuvant , Phosphate buffered saline , Recombinant hepatitis b vaccine , Antinuclear antibody , Hepatitis b vaccine , Animal experiment , Animal model , Animal tissue , Antibody titer , Anxiety , Article , Autoimmune disease , Blood cell count , Brain histology , Brain region , Cell lineage , Cognition , Controlled study , Disease exacerbation , Drug safety , Erythrocyte , Female , Forced swim test , Gliosis , Immunization , Kidney disease , Lupus like syndrome , Memory disorder , Microglia , Mouse , Nonhuman , Novel object recognition test , Proteinuria , Staircase test , Systemic lupus erythematosus , Y-maze test , Animal , Brain , Disease model , Drug effects , Immunology , Lupus erythematosus nephritis , Pathology , Pathophysiology , Animals , Antibodies , Brain , Cognition , Disease models , Female , Hepatitis b vaccines , Lupus nephritis , Mice , Proteinuria , Autoimmune/autoinflammatory syndrome induced by adjuvant (asia) , Autoimmunity , Hepatitis b vaccine , Neuro-cognitive tests , Sle , Vaccination
