Ítem
Acceso Abierto
The diagnostic performance of classical molecular tests used for detecting human papillomavirus
Título de la revista
Autores
Munoz, Marina
Camargo, Milena
Leon, Sara C. Soto-De
Rojas-Villarraga, Adriana
Sanchez, Ricardo
Jaimes, Camilo
Perez-Prados, Antonio
Patarroyo, Manuel E.
Patarroyo, Manuel A.
Archivos
Fecha
2012
Directores
ISSN de la revista
Título del volumen
Editor
Buscar en:
Métricas alternativas
Resumen
Abstract
Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k= 0.39 in the whole population; 74.1%, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated. © 2012 Elsevier B.V..
Palabras clave
Keywords
Virus DNA , Abnormal laboratory result , Adolescent , Adult , Analytic method , Article , Cancer screening , Colposcopy , Controlled study , Disease course , Female , High risk population , Human , Human cell , Hybrid capture assay 2 , Intermethod comparison , Nonhuman , Papanicolaou test , Polymerase chain reaction , Post test probability , Priority journal , Statistical parameters , Uterine cervix cancer , Uterine cervix cytology , Virus detection , Virus DNA cell DNA interaction , Wart virus , Adult , Cervix Uteri , Colposcopy , Cytological Techniques , Female , Humans , Molecular Diagnostic Techniques , Nucleic Acid Hybridization , Papillomaviridae , Papillomavirus Infections , Polymerase Chain Reaction , Prevalence , Sensitivity and Specificity , Virology , Young Adult , Human papillomavirus , Human papillomavirus , Hybrid capture assay 2 , Polymerase chain reaction , Screening , Test performance
