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Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

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Torres-Morales, Elizabeth
Taborda, Laura
Cardona, Nestor
de-la-Torre, Alejandra
Sepulveda-Arias, Juan Carlos
Patarroyo, Manuel A.
Gomez-Marin, Jorge Enrique

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2014

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Springer Verlag

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Abstract
We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN ? and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus. © 2014, Springer-Verlag Berlin Heidelberg.
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CD3 antigen , CD4 antigen , Protozoan , Toxoplasma , CD , Mononuclear , Toxoplasma gondii , CD8 antigen , Gamma interferon , Interleukin 10 , Protein p30 , ROP18 protein , Tumor necrosis factor alpha , Unclassified drug , Virulence factor , Cytokine , Leukocyte antigen , Parasite antigen , Protein serine threonine kinase , Protozoal protein , ROP18 protein , SAG1 antigen , Adult , Antigen specificity , Article , Asymptomatic disease , Carboxy terminal sequence , CD4+ T lymphocyte , CD8+ T lymphocyte , Child , Clinical article , Controlled study , Cytokine production , Ex vivo study , Female , Human , Human cell , Immune response , Immunostimulation , Male , Nonhuman , Parasite virulence , Th1 cell , Th2 cell , Toxoplasmosis , Adolescent , Cell proliferation , Chemistry , Flow cytometry , Immunology , Middle aged , Mononuclear cell , Secretion (process) , T lymphocyte subpopulation , Th1 cell , Th2 cell , Toxoplasmosis , Young adult , Adolescent , Adult , Antigens , Antigens , CD4-Positive T-Lymphocytes , CD8-Positive T-Lymphocytes , Cell Proliferation , Child , Cytokines , Female , Flow Cytometry , Humans , Leukocytes , Male , Middle Aged , Protein-Serine-Threonine Kinases , Protozoan Proteins , T-Lymphocyte Subsets , Th1 Cells , Th2 Cells , Toxoplasmosis , Young Adult , IL10 , Interferon gamma , P30 protein , Peptides , ROP18 , Th1 , Th2 , Toxoplasma , Vaccine
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