Ítem
Acceso Abierto
Concordance analysis between different methodologies used for identification of oral isolates of Candida species
Título de la revista
Autores
Zuluaga, Alejandra
Arango-Bustamante, Karen
Caceres, Diego H
Sánchez-Quitian, Zilpa A
Velásquez, Verónica
Gómez Giraldo, Beatríz Lucía
Parra-Giraldo, Claudia M
Maldonado, Natalia
Cano, Luz E
de Bedout, Catalina
Archivos
Fecha
2018
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Editor
Facultad de Salud de la Universidad del Valle
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Resumen
Abstract
Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Sixty-seven Candida isolates were tested by; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis. Results: Panfungal PCR differentiated 12 species of Candida, Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price. Conclusion: The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida. © 2018. Universidad del Valle.
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Keywords
Article , oral , matrix-assisted laser desorption-ionization , Genus , Human , Matrix assisted laser desorption ionization time of flight mass spectrometry , Mouth mucosa , Nonhuman , Polymerase chain reaction , Species identification , Adult , Candida , Clinical trial , Colombia , Comparative study , Isolation and purification , Matrix-assisted laser desorption-ionization mass spectrometry , Microbiological examination , Microbiology , Multicenter study , Polymerase chain reaction , Procedures , Thrush , Time factor , Adult , Candida , Candidiasis , Colombia , Humans , Mycological typing techniques , Polymerase chain reaction , Spectrometry , Time factors , Candida , Colombia , Diagnosis , Mass , Matrix-assisted laser desorption-ionization , Mouth mucosa , Polymerase chain reaction , Spectrometry
