Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes

Urquiza, Mauricio; Suárez, Jorge; López, Ramses; Vega, Erika; Patino, Helena; García, Javier; Patarroyo, Manuel A.; Guzmán, Fanny; Patarroyo, Manuel E.

doi:https://doi.org/10.1016/j.bbrc.2004.04.177

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Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes

https://repository.urosario.edu.co/handle/10336/25879
https://doi.org/10.1016/j.bbrc.2004.04.177

Autores

Urquiza, Mauricio

Suárez, Jorge

López, Ramses

Vega, Erika

Patino, Helena

García, Javier

Patarroyo, Manuel A.

Guzmán, Fanny

Patarroyo, Manuel E.

Fecha

2004-06-18

Editor

Elsevier

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Abstract

Epstein–Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus–cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140 nM. Of these five peptides, 11435 (181TYKRVTEKGDEHVLSLVFGK200), 11436 (201TKDLPDLRGPFSYPSLTSAQ220), and 11438 (241YFVPNLKDMFSRAVTMTAAS260) bound to a 65 kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein–Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.