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Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC

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Autores
Pinzón, Carlos Giovanni
Curtidor, Hernando
Bermudez, Adriana
Forero, Martha
Vanegas, Magnolia
Rodríguez, Jorge
Patarroyo, Manuel E.

Fecha
2008-02-06

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The Japanese Society for Vaccinology
Elsevier

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Resumen
Abstract
Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparum in vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis.
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Keywords
P. falciparum , RAMA , Rhoptry , HABP
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