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A rapid and sensitive assay for histone acetyl-transferase activity

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Ait-Si-Ali, S.
Ramírez Clavijo, Sandra Rocío
Robin, P.
Trouche, D.
Harel-Bellan, A.

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Fecha
1998-08-01

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Oxford University Press

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Abstract
Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.
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Keywords
HATs , Enzymes , Peptide
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