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Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites

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dc.creatorArévalo-Pinzón, Gabriela
dc.creatorGonzález-González, María
dc.creatorSuarez Martinez, Carlos Fernando
dc.creatorCurtidor, Hernando
dc.creatorCarabias-Sánchez, Javier
dc.creatorMuro, Antonio
dc.creatorLaBaer, Joshua
dc.creatorPatarroyo, Manuel A.
dc.creatorFuentes, Manuel
dc.creator.googleArévalo‑Pinzón, Gabrielaspa
dc.creator.googleGonzález‑González, Maríaspa
dc.creator.googleSuárez, Carlos Fernandospa
dc.creator.googleCurtidor, Hernandospa
dc.creator.googleCarabias‑Sánchez, Javierspa
dc.creator.googleMuro, Antoniospa
dc.creator.googleLaBaer, Joshuaspa
dc.creator.googlePatarroyo, Manuel Alfonsospa
dc.creator.googleFuentes, Manuelspa
dc.date.accessioned2019-09-11T17:25:36Z
dc.date.available2019-09-11T17:25:36Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractBackground: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein-protein and host-cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein-protein and host-protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification. Results: Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1. Conclusions: NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein-protein and ligand-receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax). © 2018 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1186/s12936-018-2414-2
dc.identifier.issn1475-2875
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/20256
dc.language.isoengspa
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 17
dc.relation.ispartofMalaria Journal, ISSN:1475-2875, Vol. 17 (2018)spa
dc.relation.urihttps://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2414-2spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationMueller, I., Galinski, M.R., Baird, J.K., Carlton, J.M., Kochar, D.K., Alonso, P.L., Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite (2009) Lancet Infect Dis, 9, pp. 555-566. , 19695492spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectComplementary Dnaspa
dc.subjectMsp1 Proteinspa
dc.subjectMsp8 Proteinspa
dc.subjectPlasmodium Vivax 12 Proteinspa
dc.subjectPlasmodium Vivax 41 Proteinspa
dc.subjectRap1 Proteinspa
dc.subjectRbp1A Proteinspa
dc.subjectUnclassified Drugspa
dc.subjectAmino Acid Sequencespa
dc.subjectCell Organellespa
dc.subjectControlled Studyspa
dc.subjectMerozoitespa
dc.subjectMolecular Cloningspa
dc.subjectNonhumanspa
dc.subjectNucleic Acid Programmable Protein Arrayspa
dc.subjectPlasmodium Vivaxspa
dc.subjectProtein Analysisspa
dc.subjectProtein Assemblyspa
dc.subjectProtein Expressionspa
dc.subjectProtein Microarrayspa
dc.subjectProtein Protein Interactionspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordArticlespa
dc.subject.lembMalariaspa
dc.subject.lembPlasmodiumspa
dc.subject.lembEpidemiologíaspa
dc.titleSelf-assembling functional programmable protein array for studying protein-protein interactions in malaria parasitesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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