Ítem
Acceso Abierto

Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study

Mostrar el registro sencillo de la publicación
dc.creatorVillamizar, Ximenaspa
dc.creatorHiguera, Adrianaspa
dc.creatorHerrera, Giovannyspa
dc.creatorVasquez-A, Luis Reinelspa
dc.creatorBuitron, Lorenaspa
dc.creatorMuñoz, Lina Mariaspa
dc.creatorGonzalez-C, Fabiola E.spa
dc.creatorLopez, Myriam Consuelospa
dc.creatorGiraldo, Julio Cesarspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:03:11Z
dc.date.available2020-05-26T00:03:11Z
dc.date.created2019spa
dc.description.abstractBackground: Parasitic infections, particularly those caused by protozoa, represent a considerable public health problem in developing countries. Blastocystis, Giardia duodenalis, Cryptosporidium spp. and the Entamoeba complex (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) are the most common etiological causes of intestinal parasitic infections. Methods: We carried out a descriptive cross-sectional study in school-age children attending a daycare institution in commune eight of Popayán, Cauca (Southwest Colombia). A total of 266 fecal samples were collected (258 from children and eight from pets). Blastocystis, G. duodenalis, Cryptosporidium spp. and the Entamoeba complex were identified by microscopy, quantitative real-time PCR (qPCR) and conventional PCR. The concordance of qPCR and microscopy was assessed using the Kappa index. Molecular characterization was conducted to identify Blastocystis subtypes (18S), G. duodenalis assemblages (tpi and gdh) and Cryptosporidium species/subtypes (18S and GP60). Potential associations between intestinal parasitism and sociodemographic factors were examined using bivariate analyses. Results: A total of 258 fecal samples from children were analyzed by microscopy and 255 samples were analyzed by qPCR. The prevalence of Blastocystis was between 25.19% (microscopy) and 39.22% (qPCR), that of G. duodenalis was between 8.14% (microscopy) and 10.59% (qPCR), that of Cryptosporidium spp. was estimated at 9.8% (qPCR), and that of the Entamoeba complex was between 0.39% (conventional PCR) and 0.78% (microscopy). The concordance between microscopy and qPCR was very low. Blastocystis ST1 (alleles 4, 8, and 80), ST2 (alleles 11, 12, and 15), ST3 (alleles 31, 34, 36, 38,57, and 151), and ST4 (alleles 42 and 91), G. duodenalis assemblages AII, BIII, BIV and D, C. parvum subtype IIa and C. hominis subtype IbA9G3R2 were identified. The only identified member of the Entamoeba complex corresponded to E. histolytica. No statistically significant association was identified between parasitic infection and any sociodemographic variable. Conclusion: This study revealed the usefulness of molecular methods to depict the transmission dynamics of parasitic protozoa in southwest Colombia. The presence of some of these protozoa in domestic animals may be involved in their transmission. © 2019 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s12879-019-3810-0
dc.identifier.issn14712334
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23568
dc.language.isoengspa
dc.publisherBioMed Central Ltd.spa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleBMC Infectious Diseases
dc.relation.citationVolumeVol. 19
dc.relation.ispartofBMC Infectious Diseases, ISSN:14712334, Vol.19, No.1 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85062269793&doi=10.1186%2fs12879-019-3810-0&partnerID=40&md5=fd9dec309a3f4ddf2d282cba379163b9spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAllelespa
dc.subject.keywordparasiticeng
dc.subject.keywordBlastocystisspa
dc.subject.keywordChildspa
dc.subject.keywordChilomastixspa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCross-sectional studyspa
dc.subject.keywordCryptococcus neoformansspa
dc.subject.keywordCryptosporidium parvumspa
dc.subject.keywordDemographyspa
dc.subject.keywordDiagnostic test accuracy studyspa
dc.subject.keywordDiagnostic valuespa
dc.subject.keywordDogspa
dc.subject.keywordDomestic animalspa
dc.subject.keywordEndolimax nanaspa
dc.subject.keywordEntamoeba histolyticaspa
dc.subject.keywordEscherichia colispa
dc.subject.keywordFeces analysisspa
dc.subject.keywordFemalespa
dc.subject.keywordGiardia intestinalisspa
dc.subject.keywordHumanspa
dc.subject.keywordIntermethod comparisonspa
dc.subject.keywordIntestine infectionspa
dc.subject.keywordIntestine parasitespa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMalespa
dc.subject.keywordMicroscopyspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite identificationspa
dc.subject.keywordParasite prevalencespa
dc.subject.keywordParasite transmissionspa
dc.subject.keywordParasitismspa
dc.subject.keywordParasitosisspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordProtozoonspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordAmebiasisspa
dc.subject.keywordAnimalspa
dc.subject.keywordBlastocystosisspa
dc.subject.keywordComparative studyspa
dc.subject.keywordCryptosporidiosisspa
dc.subject.keywordDogspa
dc.subject.keywordFecesspa
dc.subject.keywordGiardiasisspa
dc.subject.keywordIntestine infectionspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPet animalspa
dc.subject.keywordPreschool childspa
dc.subject.keywordPrevalencespa
dc.subject.keywordProceduresspa
dc.subject.keywordSocioeconomicsspa
dc.subject.keywordVeterinary medicinespa
dc.subject.keywordAnimalsspa
dc.subject.keywordBlastocystis infectionsspa
dc.subject.keywordChildeng
dc.subject.keywordColombiaspa
dc.subject.keywordCross-sectional studiesspa
dc.subject.keywordCryptosporidiosisspa
dc.subject.keywordDogsspa
dc.subject.keywordEntamoebiasisspa
dc.subject.keywordFecesspa
dc.subject.keywordFemalespa
dc.subject.keywordGiardiasisspa
dc.subject.keywordHumansspa
dc.subject.keywordIntestinal diseaseseng
dc.subject.keywordMalespa
dc.subject.keywordMicroscopyspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordPetsspa
dc.subject.keywordPrevalencespa
dc.subject.keywordReal-time polymerase chain reactionspa
dc.subject.keywordSocioeconomic factorsspa
dc.subject.keywordBlastocystisspa
dc.subject.keywordCryptosporidiumspa
dc.subject.keywordEntamoeba histolytica/dispar/moshkovskii complexspa
dc.subject.keywordGiardia duodenalisspa
dc.subject.keywordZoonotic diseasespa
dc.titleMolecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional studyspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
s12879-019-3810-0.pdf
Tamaño:
1.39 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos