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Tissue- specific DNA methylation profiles in newborns

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dc.contributor.advisorSteegers - Theunissen, Regine
dc.creatorGalvez Bermudez, Jubby Marcela
dc.creator.degreeMagíster en Ciencias con Énfasis en Genética Humana
dc.date.accessioned2013-01-30T17:53:45Z
dc.date.available2013-01-30T17:53:45Z
dc.date.created2011-09-02
dc.date.issued2011
dc.description.abstractExperimental and epidemiological studies demonstrate that fetal growth restriction and low birth weight enhance the risk of chronic diseases in adulthood. Derangements in tissue-specific epigenetic programming of fetal and placental tissues are a suggested mechanism of which DNA methylation is best understood. DNA methylation profiles in human tissue are mostly performed in DNA from white blood cells. The objective of this study was to assess DNA methylation profiles of IGF2 DMR and H19 in DNA derived from four tissues of the newborn. We obtained from 6 newborns DNA from fetal placental tissue (n = 5), umbilical cord CD34+ hematopoietic stem cells (HSC) and CD34- mononuclear cells (MNC) (n = 6), and umbilical cord Wharton jelly (n = 5). HCS were isolated using magnetic-activated cell separation. DNA methylation of the imprinted fetal growth genes IGF2 DMR and H19 was measured in all tissues using quantitative mass spectrometry. ANOVA testing showed tissue-specific differences in DNA methylation of IGF2 DMR (p value 0.002) and H19 (p value 0.001) mainly due to a higher methylation of IGF2 DMR in Wharton jelly (mean 0.65, sd 0.14) and a lower methylation of H19 in placental tissue (mean 0.25, sd 0.02) compared to other tissues. This study demonstrates the feasibility of the assessment of differential tissue specific DNA methylation. Although the results have to be confirmed in larger sample sizes, our approach gives opportunities to investigate epigenetic profiles as underlying mechanism of associations between pregnancy exposures and outcome, and disease risks in later life.eng
dc.description.sponsorshipErasmus MC, University Medical Center Rotterdam, The Netherlandsspa
dc.format.mimetypeapplication/pdf
dc.format.tipoDocumentospa
dc.identifier.doihttps://doi.org/10.48713/10336_4138
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/4138
dc.language.isospa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentFacultad de medicinaspa
dc.publisher.programMaestría en Ciencias con Énfasis en Genética Humanaspa
dc.rights.accesRightsinfo:eu-repo/semantics/closedAccess
dc.rights.accesoBloqueado (Texto referencial)spa
dc.rights.ccAtribución-NoComercial-SinDerivadas 2.5 Colombiaspa
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dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectEpigenomicsspa
dc.subjectUmbilical cordspa
dc.subjecthematopoietic stem cellspa
dc.subjectWharton jellyspa
dc.subjectPlacentaspa
dc.subjectImprintingspa
dc.subjectIGF2spa
dc.subjectH19spa
dc.subject.keywordEpigenomicseng
dc.subject.keywordUmbilical cordeng
dc.subject.keywordHematopoietic stem celleng
dc.subject.keywordLeukocyteseng
dc.subject.keywordWharton jellyeng
dc.subject.keywordPlacentaeng
dc.subject.keywordImprintingeng
dc.subject.keywordIGF2eng
dc.subject.keywordH19eng
dc.subject.lembComplicaciones del embarazo::Aspectos Genéticosspa
dc.subject.lembEnfermedades crónicasspa
dc.subject.lembEnfermedades genéticas en el embarazospa
dc.subject.lembMetilación de adnspa
dc.titleTissue- specific DNA methylation profiles in newbornsspa
dc.typemasterThesiseng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTesis de maestríaspa
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