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Development of peptide-based lineage-specific serology for chronic Chagas disease : Geographical and clinical distribution of epitope recognition

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dc.creatorBhattacharyya, Tapan
dc.creatorFalconar, Andrew K.
dc.creatorLuquetti, Alejandro O.
dc.creatorCostales, Jaime A.
dc.creatorGrijalva, Mario J.
dc.creatorLewis, Michael D.
dc.creatorMessenger, Louisa A.
dc.creatorTran, Trang T.
dc.creatorRamírez, Juan David
dc.creatorGuhl, Felipe
dc.creatorCarrasco, Hernan J.
dc.creatorDiosque, Patricio
dc.creatorGarcia, Lineth
dc.creatorLitvinov, Sergey V.
dc.creatorMiles, Michael A.
dc.creator.googleBhattacharyya, Tapanspa
dc.creator.googleFalconar, Andrew K.spa
dc.creator.googleLuquetti, Alejandro O.spa
dc.creator.googleCostales, Jaime A.spa
dc.creator.googleGrijalva, Mario J.spa
dc.creator.googleLewis, Michael D.spa
dc.creator.googleTran, Trang T.spa
dc.creator.googleGuhl, Felipespa
dc.creator.googleDiosque, Patriciospa
dc.creator.googleGarcia, Linethspa
dc.creator.googleLitvinov, Sergey V.spa
dc.creator.googleMiles, Michael A.spa
dc.date.accessioned2018-11-21T19:15:25Z
dc.date.available2018-11-21T19:15:25Z
dc.date.created2014
dc.date.issued2014
dc.description.abstractBackground:Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual's history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues.Methodology/Principal Findings:We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology.Conclusions/Significance:These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages. © 2014 Bhattacharyya et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0002892
dc.identifier.issnISSN 1935-2727
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18724
dc.language.isoengspa
dc.relation.citationIssueNo. 5
dc.relation.citationTitlePLoS Neglected Tropical Diseases
dc.relation.citationVolumeVol. 8
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 8/No. 5 (2014)spa
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0002892&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitation(2013) Chagas disease (American trypanosomiasis), , World Health Organization. Fact sheet N°340. Geneva: World Health Organisationspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordAntibodyeng
dc.subject.keywordProtozoaneng
dc.subject.keywordTrypanosomaeng
dc.subject.keywordAvidineng
dc.subject.keywordProtozoaneng
dc.subject.keywordBiotineng
dc.subject.keywordGlycineeng
dc.subject.keywordMacrogoleng
dc.subject.keywordParasite Antigeneng
dc.subject.keywordSynthetic Peptideeng
dc.subject.keywordEpitopeeng
dc.subject.keywordParasite Antigeneng
dc.subject.keywordPeptideeng
dc.subject.keywordProtozoon Antibodyeng
dc.subject.keywordTrypomastigote Small Surface Antigeneng
dc.subject.keywordVariant Surface Glycoproteineng
dc.subject.keywordAlgorithmeng
dc.subject.keywordAnimal Experimenteng
dc.subject.keywordAntibody Responseeng
dc.subject.keywordAntibody Specificityeng
dc.subject.keywordAntibody Specificityeng
dc.subject.keywordAntigen Recognitioneng
dc.subject.keywordBrazileng
dc.subject.keywordCell Lineageeng
dc.subject.keywordCell Lysateeng
dc.subject.keywordChagas Diseaseeng
dc.subject.keywordChronic Diseaseeng
dc.subject.keywordComparative Studyeng
dc.subject.keywordControlled Studyeng
dc.subject.keywordEcg Abnormalityeng
dc.subject.keywordEnzyme Linked Immunosorbent Assayeng
dc.subject.keywordGenotyping Techniqueeng
dc.subject.keywordGeographic Distributioneng
dc.subject.keywordHumaneng
dc.subject.keywordImmunogenicityeng
dc.subject.keywordMajor Clinical Studyeng
dc.subject.keywordMouseeng
dc.subject.keywordNonhumaneng
dc.subject.keywordNucleotide Sequenceeng
dc.subject.keywordParasite Identificationeng
dc.subject.keywordPeptide Synthesiseng
dc.subject.keywordPolymerase Chain Reactioneng
dc.subject.keywordSerologyeng
dc.subject.keywordSouth Americaeng
dc.subject.keywordTrypanosoma Cruzieng
dc.subject.keywordAmino Acid Sequenceeng
dc.subject.keywordAnimaleng
dc.subject.keywordBiologyeng
dc.subject.keywordBloodeng
dc.subject.keywordChagas Diseaseeng
dc.subject.keywordChemistryeng
dc.subject.keywordClassificationeng
dc.subject.keywordImmunologyeng
dc.subject.keywordMolecular Geneticseng
dc.subject.keywordParasitologyeng
dc.subject.keywordProcedureseng
dc.subject.keywordSerotypingeng
dc.subject.keywordTriatomaeng
dc.subject.keywordAlgorithmseng
dc.subject.keywordAmino Acid Sequenceeng
dc.subject.keywordAnimalseng
dc.subject.keywordAntibodieseng
dc.subject.keywordAntigenseng
dc.subject.keywordChagas Diseaseeng
dc.subject.keywordComputational Biologyeng
dc.subject.keywordEpitopeseng
dc.subject.keywordMiceeng
dc.subject.keywordMolecular Sequence Dataeng
dc.subject.keywordPeptideseng
dc.subject.keywordSerotypingeng
dc.subject.keywordSouth Americaeng
dc.subject.keywordTriatomaeng
dc.subject.keywordTrypanosoma Cruzieng
dc.subject.keywordVariant Surfaceeng
dc.subject.keywordGlycoproteinseng
dc.subject.lembEnfermedad de Chagasspa
dc.subject.lembTrypanosomiasisspa
dc.subject.lembCardiomiopatía Chagásicaspa
dc.titleDevelopment of peptide-based lineage-specific serology for chronic Chagas disease : Geographical and clinical distribution of epitope recognitionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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