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Synthetic peptides derived from ribosomal proteins of leishmania spp. in mucocutaneous leishmaniasis: Diagnostic usefulness

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dc.creatorFlórez M.M.spa
dc.creatorDe Oliveira C.I.spa
dc.creatorPuerta C.spa
dc.creatorGuzmán F.spa
dc.creatorAyala M.spa
dc.creatorMontoya G.spa
dc.creatorDelgado G.spa
dc.date.accessioned2020-05-25T23:58:34Z
dc.date.available2020-05-25T23:58:34Z
dc.date.created2017spa
dc.description.abstractBackground: The serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. Objective: This study was thus aimed at evaluating synthetic peptides derived from Leishmania braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA Methods: It was used 8 and 13 peptides derived from ribosomal proteins 25 and S5 respectively as antigens in order to detect IgG antibodies by ELISA in people with active MCL, Chagas disease (CH) and autoimmune disease (AID). Results: 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21.7%, 13.04%, 20% and 20%, respectively) as well as having 95%, 100%, 100% and 82.5% specificity, respectively. Conclusion: The study revealed the limited usefulness of the peptides being studied as a diagnostic tool in the conditions used here, because its low sensitivity, but it is worth highlighting that the use of peptides as antigen in the serodiagnosis of MCL may overcome the cross reaction presented with other antigens, thus avoiding false positives.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.2174/0929866524666170728143924
dc.identifier.issn0929-8665
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22889
dc.language.isoengspa
dc.publisherBentham Science Publishers B.V.spa
dc.relation.citationEndPage988
dc.relation.citationIssueNo. 10
dc.relation.citationStartPage982
dc.relation.citationTitleProtein and Peptide Letters
dc.relation.citationVolumeVol. 24
dc.relation.ispartofProtein and Peptide Letters, ISSN:9298665, Vol.24, No.10 (2017); pp. 982-988spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85041688422&doi=10.2174%2f0929866524666170728143924&partnerID=40&md5=70a23e4f16e5cdb2ac40b74da0db4436spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordImmunoglobulin g antibodyspa
dc.subject.keywordRibosome proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordRibosome proteinspa
dc.subject.keywordAntibody detectionspa
dc.subject.keywordAutoimmune diseasespa
dc.subject.keywordChagas diseasespa
dc.subject.keywordClinical articlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordInformed consentspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordMucocutaneous leishmaniasisspa
dc.subject.keywordPredictive valuespa
dc.subject.keywordProtein functionspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordSerodiagnosisspa
dc.subject.keywordSkin leishmaniasisspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAutoimmune diseasespa
dc.subject.keywordChagas diseasespa
dc.subject.keywordCross reactionspa
dc.subject.keywordImmunologyspa
dc.subject.keywordSerologyspa
dc.subject.keywordSkin leishmaniasisspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAutoimmune diseasesspa
dc.subject.keywordChagas diseasespa
dc.subject.keywordCross reactionsspa
dc.subject.keywordEnzyme-linked immunosorbent assayspa
dc.subject.keywordmucocutaneousspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordPeptidesspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordRibosomal proteinsspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordSerologic testsspa
dc.subject.keywordDiagnosisspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordMucocutaneous leishmaniasisspa
dc.subject.keywordRibosomal proteinspa
dc.subject.keywordSynthetic peptidespa
dc.titleSynthetic peptides derived from ribosomal proteins of leishmania spp. in mucocutaneous leishmaniasis: Diagnostic usefulnessspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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