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Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions

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dc.creatorArévalo-Pinzón, Gabrielaspa
dc.creatorGarzón-Ospina, Diegospa
dc.creatorPulido, Fredy A.spa
dc.creatorBermúdez, Maritzaspa
dc.creatorForero-Rodríguez, Johannaspa
dc.creatorRodríguez-Mesa, Xandy M.spa
dc.creatorReyes-Guarín, Leidy P.spa
dc.creatorSuárez, Carlos F.spa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-05-26T00:08:20Z
dc.date.available2020-05-26T00:08:20Z
dc.date.created2020spa
dc.description.abstractFollowing the injection of Plasmodium sporozoites by a female Anopheles mosquito into the dermis, they become engaged on a long journey to hepatic tissue where they must migrate through different types of cell to become established in parasitophorous vacuoles in hepatocytes. Studies have shown that proteins such as cell traversal protein for Plasmodium ookinetes and sporozoites (CelTOS) play a crucial role in cell-traversal ability. Although CelTOS has been extensively studied in various species and included in pre-clinical assays it remains unknown which P. vivax CelTOS (PvCelTOS) regions are key in its interaction with traversed or target cells (Kupffer or hepatocytes) and what type of pressure, association and polymorphism these important regions could have to improve their candidacy as important vaccine antigens. This work has described producing a recombinant PvCelTOS which was recognized by ~30% P. vivax-infected individuals, thereby confirming its ability for inducing a natural immune response. PvCelTOS' genetic diversity in Colombia and its ability to interact with HeLa (traversal cell) and/or HepG2 cell (target cell) external membrane have been assessed. One region in the PvCelTOS amino-terminal region and another in its C-terminus were seen to be participating in host-pathogen interactions. These regions had important functional constraint signals (? less than 0.3 and several sites under negative selection) and were able to inhibit specific rPvCelTOS binding to HeLa cells. This led to suggesting that sequences between aa 41–60 (40833) and 141–160 (40838) represent promising candidates for an anti-P. vivax subunit-based vaccine. © Copyright © 2020 Arévalo-Pinzón, Garzón-Ospina, Pulido, Bermúdez, Forero-Rodríguez, Rodríguez-Mesa, Reyes-Guarín, Suárez and Patarroyo.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fcimb.2020.00119
dc.identifier.issn22352988
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24078
dc.language.isoengspa
dc.publisherFrontiers Media S.A.spa
dc.relation.citationTitleFrontiers in Cellular and Infection Microbiology
dc.relation.citationVolumeVol. 10
dc.relation.ispartofFrontiers in Cellular and Infection Microbiology, ISSN:22352988, Vol.10,(2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85083072127&doi=10.3389%2ffcimb.2020.00119&partnerID=40&md5=d882e777db352751c0bc6a3ec43a0f32spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAntigenicityspa
dc.subject.keywordArticlespa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordBlood samplingspa
dc.subject.keywordCell selectionspa
dc.subject.keywordCell surfacespa
dc.subject.keywordClinical articlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCrystal structurespa
dc.subject.keywordDna extractionspa
dc.subject.keywordDna purificationspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordFlow cytometryspa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGene frequencyspa
dc.subject.keywordGene sequencespa
dc.subject.keywordGenetic polymorphismspa
dc.subject.keywordGenetic selectionspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordHost pathogen interactionspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmunofluorescence testspa
dc.subject.keywordIsotope labelingspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPlasmidspa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPlasmodium vivax malariaspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein expressionspa
dc.subject.keywordProtein structurespa
dc.subject.keywordSequence analysisspa
dc.subject.keywordSingle nucleotide polymorphismspa
dc.subject.keywordSporozoitespa
dc.subject.keywordWestern blottingspa
dc.subject.keywordCeltosspa
dc.subject.keywordMalariaspa
dc.subject.keywordMulti-epitope multi-stage vaccinespa
dc.subject.keywordPspa
dc.subject.keywordVivaxspa
dc.subject.keywordSporozoitespa
dc.titlePlasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactionsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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