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The Mycobacterium tuberculosis membrane protein Rv2560 - Biochemical and functional studies

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dc.creatorPlaza, David F.spa
dc.creatorCurtidor, Hernandospa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorChapeton?Montes, Julie A.spa
dc.creatorReyes, Claudiaspa
dc.creatorBarreto, Josespa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-05-26T00:08:06Z
dc.date.available2020-05-26T00:08:06Z
dc.date.created2007spa
dc.description.abstractThe characterization of membrane proteins having no identified function in Mycobacterium tuberculosis is important for a better understanding of the biology of this pathogen. In this work, the biological activity of the Rv2560 protein was characterized and evaluated. Primers used in PCR and RT-PCR assays revealed that the gene encoding protein Rv2560 is present in M. tuberculosis complex strains, but transcribed in only some of them. Sera obtained from rabbits inoculated with polymer peptides from this protein recognized a 33 kDa band in the M. tuberculosis lysate and a membrane fraction corresponding to the predicted molecular mass (33.1 kDa) of this protein. Immunoelectron microscopy analysis found this protein on the mycobacterial membrane. Sixteen peptides covering its entire length were chemically synthesized and tested for their ability to bind to A549 and U937 cells. Peptide 11024 (121VVALSDRATTAYTNTSGVSS140) showed high specific binding to both cell types (dissociation constants of 380 and 800 nm, respectively, and positive receptor-ligand interaction cooperativity), whereas peptide 11033 (284LIGIPVAALIHVYTYRKLSGG304) displayed high binding activity to A549 cells only. Cross-linking assays showed the specific binding of peptide 11024 to a 54 kDa membrane protein on U937. Invasion inhibition assays, in the presence of shared high-activity binding peptide identified for U937 and A549 cells, presented maximum inhibition percentages of 50.53% and 58.27%, respectively. Our work highlights the relevance of the Rv2560 protein in the M. tuberculosis invasion process of monocytes and epithelial cells, and represents a fundamental step in the rational selection of new antigens to be included as components in a multiepitope, subunit-based, chemically synthesized, antituberculosis vaccine. © 2007 The Authors.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/j.1742-4658.2007.06153.x
dc.identifier.issn1742464X
dc.identifier.issn14321033
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24055
dc.language.isoengspa
dc.relation.citationEndPage6364
dc.relation.citationIssueNo. 24
dc.relation.citationStartPage6352
dc.relation.citationTitleFEBS Journal
dc.relation.citationVolumeVol. 274
dc.relation.ispartofFEBS Journal, ISSN:1742464X, 14321033, Vol.274, No.24 (2007); pp. 6352-6364spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-36749082971&doi=10.1111%2fj.1742-4658.2007.06153.x&partnerID=40&md5=7b8a0cce8255bf4095e41ce402a4ae8dspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordMembrane proteinspa
dc.subject.keywordproteineng
dc.subject.keywordmatrix-assisted laser desorption-ionizationeng
dc.subject.keywordUnclassified drugspa
dc.subject.keywordArticlespa
dc.subject.keywordBiochemistryspa
dc.subject.keywordBiological activityspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCross linkingspa
dc.subject.keywordEpithelium cellspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordMonocytespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordRabbitspa
dc.subject.keywordReverse transcription polymerase chain reactionspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordBlottingeng
dc.subject.keywordCell lineeng
dc.subject.keywordCircular dichroismspa
dc.subject.keywordDnaeng
dc.subject.keywordFlow cytometryspa
dc.subject.keywordHumansspa
dc.subject.keywordImmune seraspa
dc.subject.keywordImmunizationspa
dc.subject.keywordMembrane proteinsspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordRabbitsspa
dc.subject.keywordSequence analysiseng
dc.subject.keywordSequence analysiseng
dc.subject.keywordSpectrometryeng
dc.subject.keywordU937 cellsspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordOryctolagus cuniculusspa
dc.subject.keywordHigh-activity binding peptidespa
dc.subject.keywordInvasion inhibitionspa
dc.subject.keywordMycobacterium tuberculosis- host cell interactionspa
dc.subject.keywordRv2560 membrane proteinspa
dc.titleThe Mycobacterium tuberculosis membrane protein Rv2560 - Biochemical and functional studiesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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