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Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region

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dc.creatorCubides, Juan Ricardo
dc.creatorCamargo-Ayala, Paola Andrea
dc.creatorNiño, Carlos Hernando
dc.creatorGarzón‑Ospina, Diego
dc.creatorOrtega‑Ortegón, Anggie
dc.creatorOspina‑Cantillo, Estefany
dc.creatorOrduz‑Durán, María Fernanda
dc.creatorPatarroyo, Manuel Elkin
dc.creatorPatarroyo, Manuel A.
dc.creator.googleCubides, J.R., Camargo-Ayala, P.A., Niño, C.H., Garzón-Ospina, D., Ortega-Ortegón, A., Ospina-Cantillo, E., Orduz-Durán, M.F., Patarroyo, M.E., Patarroyo, M.A.spa
dc.date.accessioned2019-10-03T12:35:49Z
dc.date.available2019-10-03T12:35:49Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractBackground: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region. Methods: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained. Conclusion: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage. © 2018 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1186/s12936-018-2286-5
dc.identifier.issn1475-2875
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20382
dc.language.isoengspa
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 17
dc.relation.ispartofMalaria Journal, ISSN:1475-2875, Vol. 17 (2018)spa
dc.relation.urihttps://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2286-5spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitation(2016) World Malaria Report 2016, , WHO World Health Organization Genevaspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectMalaria Vaccinespa
dc.subjectColombiaspa
dc.subjectCrt O Genespa
dc.subjectDhfr Genespa
dc.subjectDhps Genespa
dc.subjectDisease Surveillancespa
dc.subjectElectrophoresisspa
dc.subjectEndemic Diseasespa
dc.subjectGenespa
dc.subjectGene Locationspa
dc.subjectGene Mutationspa
dc.subjectGene Sequencespa
dc.subjectGenetic Associationspa
dc.subjectGenetic Polymorphismspa
dc.subjectGenetic Resistancespa
dc.subjectGenetic Selectionspa
dc.subjectGenetic Variabilityspa
dc.subjectHaplotypespa
dc.subjectHumanspa
dc.subjectMalaria Controlspa
dc.subjectMdr1 Genespa
dc.subjectNonhumanspa
dc.subjectNucleotide Sequencespa
dc.subjectPlasmodium Vivaxspa
dc.subjectPlasmodium Vivax Malariaspa
dc.subjectPolymerase Chain Reactionspa
dc.subjectVacuna contra la malariaspa
dc.subjectCrt O génicaspa
dc.subjectColombiaspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordArticlespa
dc.subject.lembMalariaspa
dc.subject.lembPlasmodiumspa
dc.titleSimultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian regionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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