Ítem
Solo Metadatos

Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis

Mostrar el registro sencillo de la publicación
dc.creatorSeret, Guillaumespa
dc.creatorCañas, Felipespa
dc.creatorCostanzo, Laurence Pougnet-Dispa
dc.creatorHanrotel-Saliou, Catherinespa
dc.creatorJousse-Joulin, Sandrinespa
dc.creatorLe Meur, Yannickspa
dc.creatorSaraux, Alainspa
dc.creatorValeri, Antoinespa
dc.creatorPutterman, Chaimspa
dc.creatorYouinou, Pierrespa
dc.creatorRojas-Villarraga, Adrianaspa
dc.creatorAnaya, Juan-Manuelspa
dc.creatorRenaudineau, Yvesspa
dc.date.accessioned2020-06-11T13:21:52Z
dc.date.available2020-06-11T13:21:52Z
dc.date.created2015-07spa
dc.description.abstractAnti-membrane autoantibodies (MbA) have been reported in sera from patients with lupus nephritis (LN) but the targets of the MbA remain to be explored, which is the aim of the current study. Sera were collected from 40 patients with LN determined by renal biopsy, and from 30 systemic lupus erythematosus (SLE) patients without clinical evidence of LN. Thirty autoimmune disease control patients (rheumatoid arthritis, Sjogren's syndrome and systemic sclerosis), and 30 healthy controls were also included. Using flow cytometry, the presence of anti-MbA was explored revealing that IgG anti-MbA positivity was associated with LN (62.5% vs 13.3%) when compared to non-LN SLE patients, autoimmune disease patients (6.7%) and healthy controls (0%). Next, using purified plasma membrane fractions from human embryonic kidney (HEK) cells, the more prominent targets and their occurrence rates were located at 50 kDa, 60/65 kDa, 90 kDa, 110 kDa, 180 kDa and 220 kDa. Alpha-actinin (110 kDa) autoAb was characterized as a major target in LN patients positive for anti-MbA, and anti-MbA binding activity was reduced (36.9 +/- 13.7%) in the presence of alpha-actinin. Laminin (200 kDa) was also characterized as a minor target, which was not the case for annexin A2 (36 kDa). Finally, anti-MbA IgG subclass analysis indicated a predominance of IgG2. In conclusion, IgG anti-MbA were detected at high levels in LN patients supporting a primary pathogenic role for anti-MbA and anti-MbA/alpha-actinin+ in LN that needs further research. (C) 2015 Elsevier Ltd. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jaut.2015.05.009
dc.identifier.issn0896-8411
dc.identifier.issn1095-9157
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24939
dc.language.isoeng
dc.publisherJournal of Autoimmunityspa
dc.relation.citationEndPage61
dc.relation.citationStartPage54
dc.relation.citationTitleJournal of Autoimmunity
dc.relation.citationVolumeVol. 61
dc.relation.ispartofJournal of Autoimmunity, ISSN: 0896-8411;1095-9157, Vol.61, No. (2015-07); pp. 54-61spa
dc.relation.urihttps://app.dimensions.ai/details/publication/pub.1004921433spa
dc.rights.accesRightsinfo:eu-repo/semantics/closedAccess
dc.rights.accesoBloqueado (Texto referencial)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordActininspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordAgedspa
dc.subject.keywordAutoantibodiesspa
dc.subject.keywordCell Membranespa
dc.subject.keywordCellsspa
dc.subject.keywordCulturedspa
dc.subject.keywordEnzyme-Linked Immunosorbent Assayspa
dc.subject.keywordFemalespa
dc.subject.keywordFlow Cytometryspa
dc.subject.keywordHEK293 Cellsspa
dc.subject.keywordHumansspa
dc.subject.keywordImmunoglobulin Gspa
dc.subject.keywordLupus Erythematosusspa
dc.subject.keywordSystemicspa
dc.subject.keywordLupus Nephritisspa
dc.subject.keywordMalespa
dc.subject.keywordMesangial Cellsspa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordYoung Adultspa
dc.titleAnti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos