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Peptides Derived of Kunitz-Type Serine Protease Inhibitor as Potential Vaccine Against Experimental Schistosomiasis

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dc.creatorHernández-Goenaga, Juanspa
dc.creatorLópez-Abán, Juliospa
dc.creatorProtasio, Anna V.spa
dc.creatorSantiago, Belén Vicentespa
dc.creatordel Olmo, Estherspa
dc.creatorVanegas, Magnoliaspa
dc.creatorFernández-Soto, Pedrospa
dc.creatorPatarroyo, Manuel A.
dc.creatorMuro, Antoniospa
dc.date.accessioned2020-05-26T00:08:29Z
dc.date.available2020-05-26T00:08:29Z
dc.date.created2019spa
dc.description.abstractSchistosomiasis is a significant public health problem in sub-Saharan Africa, China, Southeast Asia, and regions of South and Central America affecting about 189 million people. Kunitz-type serine protease inhibitors have been identified as important players in the interaction of other flatworm parasites with their mammalian hosts. They are involved in host blood coagulation, fibrinolysis, inflammation, and ion channel blocking, all of them critical biological processes, which make them interesting targets to develop a vaccine. Here, we evaluate the protective efficacy of chemically synthesized T- and B-cell peptide epitopes derived from a kunitz protein from Schistosoma mansoni. Putative kunitz-type protease inhibitor proteins were identified in the S. mansoni genome, and their expression was analyzed by RNA-seq. Gene expression analyses showed that the kunitz protein Smp_147730 (Syn. Smp_311670) was dramatically and significantly up-regulated in schistosomula and adult worms when compared to the invading cercariae. T- and B-cell epitopes were predicted using bioinformatics tools, chemically synthesized, and formulated in the Adjuvant Adaptation (ADAD) vaccination system. BALB/c mice were vaccinated and challenged with S. mansoni cercariae. Kunitz peptides were highly protective in vaccinated BALB/c mice showing significant reductions in recovery of adult females (89–91%) and in the numbers of eggs trapped in the livers (77–81%) and guts (57–77%) of mice. Moreover, liver lesions were significantly reduced in vaccinated mice (64–65%) compared to infected control mice. The vaccination regime was well-tolerated with both peptides. We propose the use of these peptides, alone or in combination, as reliable candidates for vaccination against schistosomiasis. © Copyright © 2019 Hernández-Goenaga, López-Abán, Protasio, Vicente Santiago, del Olmo, Vanegas, Fernández-Soto, Patarroyo and Muro.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fimmu.2019.02498
dc.identifier.issn16643224
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24088
dc.language.isoengspa
dc.publisherFrontiers Media S.A.spa
dc.relation.citationTitleFrontiers in Immunology
dc.relation.citationVolumeVol. 10
dc.relation.ispartofFrontiers in Immunology, ISSN:16643224, Vol.10,(2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85075115022&doi=10.3389%2ffimmu.2019.02498&partnerID=40&md5=b91880e23c3ec46ca08bf9fb45958552spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCD1 antigenspa
dc.subject.keywordEpitopespa
dc.subject.keywordIon channelspa
dc.subject.keywordSerine proteinase inhibitorspa
dc.subject.keywordVaccinespa
dc.subject.keywordAdultspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAnimal modelspa
dc.subject.keywordAnimal tissuespa
dc.subject.keywordArticlespa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordBiomphalaria glabrataspa
dc.subject.keywordCercariaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDrug efficacyspa
dc.subject.keywordEchinococcus multilocularisspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFasciola hepaticaspa
dc.subject.keywordFemalespa
dc.subject.keywordFibrinolysisspa
dc.subject.keywordGene expressionspa
dc.subject.keywordInflammationspa
dc.subject.keywordMolecular geneticsspa
dc.subject.keywordMousespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPeptide synthesisspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordReversed phase high performance liquid chromatographyspa
dc.subject.keywordSchistosoma granulosusspa
dc.subject.keywordSchistosoma haematobiumspa
dc.subject.keywordSchistosoma japonicumspa
dc.subject.keywordSchistosoma mansonispa
dc.subject.keywordSchistosomiasisspa
dc.subject.keywordSchistosomulumspa
dc.subject.keywordStereomicroscopyspa
dc.subject.keywordUpregulationspa
dc.subject.keywordVaccinationspa
dc.subject.keywordADAD vaccination systemspa
dc.subject.keywordHelminth vaccinesspa
dc.subject.keywordImmunomodulator AA0029spa
dc.subject.keywordKunitz-type proteinsspa
dc.subject.keywordSchistosoma mansonispa
dc.subject.keywordSynthetic peptidespa
dc.titlePeptides Derived of Kunitz-Type Serine Protease Inhibitor as Potential Vaccine Against Experimental Schistosomiasisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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