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Evaluation of a Histoplasma antigen lateral flow assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS

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dc.creatorCáceres, Diego H.spa
dc.creatorGómez, Beatriz L.spa
dc.creatorTobón, Angela M.spa
dc.creatorChiller, Tom M.spa
dc.creatorLindsley, Mark D.spa
dc.date.accessioned2020-05-25T23:57:14Z
dc.date.available2020-05-25T23:57:14Z
dc.date.created2020spa
dc.description.abstractBackground: Progressive disseminated histoplasmosis (PDH) is an important cause of mortality in persons living with HIV (PLHIV), especially in countries where patients have limited access to antiretroviral therapies and diagnostic testing. Objective: A lateral flow assay (LFA) to detect Histoplasma capsulatum antigen in serum developed by MiraVista® was evaluated. Methods: We tested 75 serum samples: 24 from PLHIV and culture-proven PDH and 51 from PLHIV with other fungal and bacterial infections as well as people without HIV. LFA devices were read manually (read by eye) and by an automated reader. Results: When the LFA was read manually, sensitivity was 96% and specificity was 90%. When an automated reader was used, sensitivity was 92% and specificity was 94%. The Kappa index comparing manual and automated reader was 0.90. Cross-reactions were observed principally in samples from patients with proven diagnosis of paracoccidioidomycosis. Conclusions: The MiraVista® Diagnostics Histoplasma antigen LFA had high analytical performance and good agreement between manual and automated reader. This LFA allows Histoplasma antigen testing with minimal laboratory equipment and infrastructure requirements. © 2019 Blackwell Verlag GmbHeng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/myc.13023
dc.identifier.issn09337407
dc.identifier.issn14390507
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22635
dc.language.isoengspa
dc.publisherBlackwell Publishing Ltdspa
dc.relation.citationEndPage144
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage139
dc.relation.citationTitleMycoses
dc.relation.citationVolumeVol. 63
dc.relation.ispartofMycoses, ISSN:09337407, 14390507, Vol.63, No.2 (2020); pp. 139-144spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85075746317&doi=10.1111%2fmyc.13023&partnerID=40&md5=b99e820176b72a088575a2cc7552a84bspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordFungus antigenspa
dc.subject.keywordHistoplasma capsulatum antigenspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAids patientspa
dc.subject.keywordAntigen detectionspa
dc.subject.keywordArticlespa
dc.subject.keywordColombianspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCross reactionspa
dc.subject.keywordDiagnostic accuracyspa
dc.subject.keywordDiagnostic test accuracy studyspa
dc.subject.keywordHistoplasma capsulatumspa
dc.subject.keywordHistoplasmosisspa
dc.subject.keywordHumanspa
dc.subject.keywordImmunoassayspa
dc.subject.keywordLateral flow assayspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProgressive disseminated histoplasmosisspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordSouth american blastomycosisspa
dc.titleEvaluation of a Histoplasma antigen lateral flow assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDSspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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